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SATB1 Antibody (PA5-30162) in ICC/IF
Immunofluorescence analysis of SATB1 was performed using 70% confluent log phase MDA-MB-231 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with SATB1 Polyclonal Antibody (Product # PA5-30162) at 1:500 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody (Product # A27034) at ... View More
产品信息
PA5-30162
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fragment corresponding to a region within amino acids 39 and 379 of Human SATB1
偶联物
形式
Liquid
浓度
0.78 mg/mL
纯化类型
Antigen affinity chromatography
保存液
0.1M tris glycine, pH 7, with 20% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2547636
产品详细信息
Recommended positive controls: Jurkat, MDA-MB-231, THP-1, mouse thymus, mouse brain, rat brain.
Predicted reactivity: Mouse (100%), Rat (100%), Chicken (98%), Rhesus Monkey (100%), Bovine (98%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
SATB1 (DNA-binding protein SATB1) is a crucial silencing factor that contributes to the initiation of X inactiviation mediated by Xist RNA that occurs during embryogenesis and in lymphoma. SATB1 binds to DNA at special AT-rich sequences, the consensus SATB1-binding sequence, and at nuclear matrix- or scaffold-associated regions. It is thought to recognize the sugar-phosphate structure of double-stranded DNA. SATB1 is also a transcriptonal repressor controlling nuclear and viral gene expression in a phosphorylated and acetylated status-dependent manner by binding to matrix attachment regions of DNA and inducing a local chromatin-loop remodeling. SATB1 modulates the genes that are essential in the maturatio of the immune T-cell CD8SP from thymocytes. Alu-like motifs and SATB1-binding sites provide an unique chromatin context which seems preferentially targeted by the HIV-1 integration machinery. Moreover, HIV-1 Tat may overcome SATB1-mediated repression of IL1 and IL2RA in T-cells by binding to the same domain as HDAC1.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DNA-binding protein SATB1; special AT-rich sequence binding protein 1; special AT-rich sequence binding protein 1 (binds to nuclear matrix/scaffold-associating DNA); Special AT-rich sequence-binding protein 1
基因别名: 2610306G12Rik; AW413156; SATB1
UniProt ID: (Human) Q01826, (Mouse) Q60611
Entrez Gene ID: (Human) 6304, (Mouse) 20230, (Rat) 316164
RNA polymerase II regulatory region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
chromatin binding
double-stranded DNA binding
protein binding
DNA binding
transcription factor activity, sequence-specific DNA binding
helix-turn-helix transcription factor
DNA-binding transcription factor
Lambda repressor-like transcription factor
gene-specific transcriptional regulator
negative regulation of transcription from RNA polymerase II promoter
chromatin organization
chromatin remodeling
transcription, DNA-templated
cellular component disassembly involved in execution phase of apoptosis
epidermis development
viral process
histone methylation
CD4-positive, alpha-beta T cell differentiation
CD8-positive, alpha-beta T cell differentiation
activated T cell proliferation
reflex
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
chromatin modification
T cell activation
