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SCF Antibody (PA5-47290) in ICC/IF
Immunocytochemistry analysis of SCF in immersion fixed canine peripheral blood mononuclear cells (PBMCs). Samples were incubated in SCF polyclonal antibody (Product # PA5-47290) using a dilution of 15 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane.
产品信息
PA5-47290
应用
建议稀释比
已发表文章
产品规格
种属反应
Dog
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E. coli-derived recombinant canine SCF/c-kit Ligand Lys26-Ala190
偶联物
形式
Lyophilized
浓度
0.2 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 5% trehalose
内含物
No Preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2608095
产品详细信息
In sandwich ELISAs, less than 20% cross-reactivity with recombinant feline SCF is observed, less than 8% cross-reactivity with recombinant human SCF is observed, and less than 0.5% cross-reactivity with recombinant mouse SCF is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
靶标信息
Stem cell factor (SCF) is the ligand of the c-Kit oncogene and is expressed by various structural and inflammatory cells in the airways. Binding of SCF by the c-Kit receptor leads to homodimerization of the receptor and the activation of signalling pathways such as PI-3, PLC-gamma, Jak/STAT, and MAP kinase pathways. SCF expression leads to the induction of mast cell survival and the expression and release of histamine, pro-inflammatory cytokines and chemokines. The inhibition of the SCF/c-Kit pathway leads to a decrease in histamine levels, mast cell and eosinophil infiltration, IL-4 production and airway hyperresponsiveness, suggesting this pathway may be a useful therapeutic target in inflammatory diseases such as asthma. At least two isoforms of SCF are known to exist.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: c-Kit ligand; DKFZp686F2250; H-SCF; Kit ligand; kit-ligand; M-SCF; Mast cell growth factor; MGF; SCF; Stem cell factor; stem cell factor, CSF
基因别名: 710-712; CSF; KITLG; MGF
UniProt ID: (Dog) Q06220
Entrez Gene ID: (Dog) 403507
