Invitrogen

SCRIB Polyclonal Antibody

SCRIB Antibody in Immunocytochemistry (ICC/IF)

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SCRIB Antibody (PA5-116752) in ICC/IF

Immunofluorescence analysis of SCRIB was performed using 70% confluent log phase HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.01% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SCRIB Polyclonal Antibody (Product # PA5-116752) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:... View More

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SCRIB Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HEK-293 cells were transfected with SCRIB siRNA and decrease in signal intensity was observed in Western Blot application using Anti-SCRIB Polyclonal Antibody (Product # PA5-116752). {KD}

产品信息

PA5-116752

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

免疫细胞化学 (ICC/IF)

1:100

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 1100-1400 of human SCRIB (NP_8743653)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

3.91 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.05% ProClin 300

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2901383

产品详细信息

Positive Samples: A-549, 293T

Immunogen sequence: ELCIQKAPGE RLGISIRGGA RGHAGNPRDP TDEGIFISKV SPTGAAGRDG RLRVGLRLLE VNQQSLLGLT HGEAVQLLRS VGDTLTVLVC DGFEASTDAA LEVSPGVIAN PFAAGIGHRN SLESISSIDR ELSPEGPGKE KELPGQTLHW GPEATEAAGR GLQPLKLDYR ALAAVPSAGS VQRVPSGAAG GKMAESPCSP SGQQPPSPPS PDELPANVKQ AYRAFAAVPT SHPPEDAPAQ PPTPGPAASP EQLSFRERQK YFELEVRVPQ AEGPPKRVSL VGADDLRKMQ EEEARKLQQK R

靶标信息

SCRIB is a cytoplasmic multimodular scaffold protein targeted to epithelial adherens junctions and neuronal presynaptic compartments. SCRIB and its orthologs in vertebrates and invertebrates participate in cell polarization.

仅用于科研。不用于诊断过程。未经明确授权不得转售。