Invitrogen

SCRIB Polyclonal Antibody

SCRIB Antibody in Immunocytochemistry (ICC/IF)

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SCRIB Antibody (PA5-142476) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of SCRIB in MCF7 cells using SCRIB Polyclonal Antibody (Product # PA5-142476). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cell junction staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

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产品信息

PA5-142476

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-3 µg/mL

免疫细胞化学 (ICC/IF)

10 µg/mL

产品规格

种属反应

Human

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

Peptide with sequence C-PEGPGKEKELPGQ.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Ammonium sulfate precipitation

保存液

TBS, pH 7.3, with 0.5% BSA

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2933124

产品详细信息

Additional Information: This antibody is expected to recognize both reported isoforms (NP_874365.3; NP_056171.3).

This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:1,000.

靶标信息

SCRIB is a cytoplasmic multimodular scaffold protein targeted to epithelial adherens junctions and neuronal presynaptic compartments. SCRIB and its orthologs in vertebrates and invertebrates participate in cell polarization.

仅用于科研。不用于诊断过程。未经明确授权不得转售。