Invitrogen

SCRIB Polyclonal Antibody

SCRIB Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 6

SCRIB Antibody (PA5-54821) in ICC/IF

Immunofluorecent analysis of SCRIB in human cell line U-2 OS using SCRIB Polyclonal Antibody (Product # PA5-54821). Staining shows localization to nucleoplasm, plasma membrane and cell junctions.

Product Image Gallery

SCRIB Antibody — Relative expression in western blot: Antibody specificity was demonstrated by detection of known differential basal expression of the target across tissue/cell models. Expression of SCRIB was observed specifically in MCF-7 cells and in Caco-2 cells using anti-SCRIB Polyclonal Antibody (Product # PA5-54821) in western blot. The relative expression levels of SCRIB within each cell line is shown using RNA-Seq. {RE}

产品信息

PA5-54821

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.04-0.4 µg/mL

查看2篇已发表文章 2篇已发表文章

免疫细胞化学 (ICC/IF)

0.25-2 µg/mL

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein corresponding to Human SCRIB. Recombinant protein control fragment (Product #RP-93933).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.40 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2647030

产品详细信息

Immunogen sequence: VSVIQFLEAP IGDEDAEEAA AEKRGLQRRA TPHPSELKVM KRSIEGRRSE ACPCQPDSGS PLPAEEEKRL SAESGLSEDS RPSASTVS

Highest antigen sequence identity to the following orthologs: Mouse - 75%, Rat - 74%.

靶标信息

SCRIB is a cytoplasmic multimodular scaffold protein targeted to epithelial adherens junctions and neuronal presynaptic compartments. SCRIB and its orthologs in vertebrates and invertebrates participate in cell polarization.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Native holdup (nHU) to measure binding affinities from cell extracts.

Science advances

Zambo B,Morlet B,Negroni L,Trave G,Gogl G

Published figure using SCRIB polyclonal antibody (Product # PA5-54821) in Western Blot

Wed Dec 21 00:00:00 UTC 2022

Quantitative fragmentomics allow affinity mapping of interactomes.

Nature communications

Gogl G,Zambo B,Kostmann C,Cousido-Siah A,Morlet B,Durbesson F,Negroni L,Eberling P,Jané P,Nominé Y,Zeke A,Østergaard S,Monsellier É,Vincentelli R,Travé G

PA5-54821 was used in Western Blotting to show that interactome hijacking by the viral PBM of human papillomavirus E6 oncoprotein substantially impacts the host cell proteome beyond immediate E6 binders, illustrating the complex system-wide relationship between interactome and function.