Invitrogen

SCXA Polyclonal Antibody

SCXA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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SCXA Antibody (PA5-23943) in IHC (P)

Immunohistochemistry analysis of SCXA in paraffin-embedded Human breast carcinoma tissue. Samples were incubated with SCXA polyclonal antibody (Product # PA5-23943) using a dilution of 1:100 for 1 hour at room temperature followed by undiluted CRF Anti-Polyvalent HRP Polymer antibody. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH 9 0)... View More

SCXA Antibody in Immunocytochemistry (ICC/IF)

SCXA Antibody in Western Blot, ChIP Assay (WB, ChIP)

Product Image Gallery

SCXA Antibody — Figure 3 cANGPTL4 regulates COL1A2 and COL3A1 expression in fibroblasts via a bHLH-dependent signaling mechanism. ( a ) Immunoblot analysis of indicated proteins from fibroblasts treated with cANGPTL4 (12 ug/mL) in the presence or absence of TGFbeta1 (10 ng/mL). beta-tubulin, as loading control, was from the same samples. ( b,d ) Chromatin immunoprecipitation (ChIP) of SBE sites in the human COL1A2 gene promoter region and E-box sites in the human COL1A2 and COL3A1 gene promoter regions using phospho-Smad3 antibodies and scleraxis (ScxA) antibodies respectively. The gene sequences spanning the SBE and E-box sites and a random control sequence were analyzed by PCR from immunoprecipitated chromatin from fibroblasts treated with TGFbeta1 in the presence or absence of cANGPTL4 (cANG). Pre-immune serum was used as a control. PCR was performed using chromatin before immunoprecipitation as input. M, 100 bp DNA ladder. ( c ) A schematic diagram showing the relative positions of Enhancer boxes (E-Boxes) and Smad binding elements (SBE) on the human COL1A2 and COL3A1 proximal promoters. Sequences of E-Boxes and SBE are shown. ChIP PCR primers designed specifically to bind scleraxis along this proximal promoter region are indicated. ( e ) Relative mRNA expression of ID3 in fibroblasts treated with 12 ug/mL of cANGPTL4 and lysed for mRNA at the indicated time points post-treatment. ( f ) Relative mRNA expression of ID3 in control (F ctrl ) and ID3-knockdown (F [delta]ID3 ) fibroblasts treated {TM}

产品信息

PA5-23943

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看6篇已发表文章 6篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100

免疫细胞化学 (ICC/IF)

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染色质免疫沉淀实验 (ChIP)

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产品规格

种属反应

Human, Mouse

已发表种属

Chicken, Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 172-201 amino acids from the C-terminal region of human SCXA

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.45 mg/mL

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2541443

产品详细信息

This antibody is predicted to react with chicken based on sequence homology.

靶标信息

Enables DNA-binding transcription activator activity, RNA polymerase II-specific and bHLH transcription factor binding activity. Contributes to E-box binding activity. Involved in positive regulation of transcription by RNA polymerase II. Located in nucleus. Part of transcription regulator complex.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

TGFβ signaling is required for sclerotome resegmentation during development of the spinal column in Gallus gallus.

Developmental biology

Clayton SW,Angermeier A,Halbrooks JE,McCardell R,Serra R

PA5-23943 was used in Western Blotting to propose that TGFβ signaling regulates rostro-caudal polarity and subsequent resegmentation in sclerotome during spinal column development.

Mon Aug 01 00:00:00 UTC 2022

The Effect of L-Ascorbic Acid and Serum Reduction on Tenogenic Differentiation of Human Mesenchymal Stromal Cells.

International journal of stem cells

Bochon K,Zielniok K,Gawlak M,Zawada K,Zarychta-Wiśniewska W,Siennicka K,Struzik S,Pączek L,Burdzińska A

PA5-23943 was used in Western Blot, Immunocytochemistry to investigate if serum reduction, L-ascorbic acid supplementation or a combination of both factors can induce tenogenic differentiation of human adipose-derived MSCs (ASCs).

Sun Feb 28 00:00:00 UTC 2021

The Effect of L-Ascorbic Acid and Serum Reduction on Tenogenic Differentiation of Human Mesenchymal Stromal Cells.

International journal of stem cells

Bochon K,Zielniok K,Gawlak M,Zawada K,Zarychta-Wiśniewska W,Siennicka K,Struzik S,Pączek L,Burdzińska A

Sun Feb 28 00:00:00 UTC 2021

The Effect of L-Ascorbic Acid and Serum Reduction on Tenogenic Differentiation of Human Mesenchymal Stromal Cells.

International journal of stem cells

Bochon K,Zielniok K,Gawlak M,Zawada K,Zarychta-Wiśniewska W,Siennicka K,Struzik S,Pączek L,Burdzińska A

Sun Feb 28 00:00:00 UTC 2021

Copper Does Not Induce Tenogenic Differentiation but Promotes Migration and Increases Lysyl Oxidase Activity in Adipose-Derived Mesenchymal Stromal Cells.

Stem cells international

Milewska M,Burdzińska A,Zielniok K,Siennicka K,Struzik S,Zielenkiewicz P,Pączek L

PA5-23943 was used in Western Blot to verify whether copper supplementation may have a positive effect on the properties of human adipose tissue-derived MSCs (hASCs) which potentially can contribute to improvement of tendon healing.

Wed Apr 13 00:00:00 UTC 2022

The Transcription Factor SCX is a Potential Serum Biomarker of Fibrotic Diseases.

International journal of molecular sciences

Ramírez-Aragón M,Hernández-Sánchez F,Rodríguez-Reyna TS,Buendía-Roldán I,Güitrón-Castillo G,Núñez-Alvarez CA,Hernández-Ramírez DF,Benavides-Suárez SA,Esquinca-González A,Torres-Machorro AL,Mendoza-Milla C

PA5-23943 was used in Western Blotting to propose the bHLH transcription factor scleraxis (SCX) could be a candidate biomarker of fibrotic diseases.

Thu Jul 16 00:00:00 UTC 2020

The Transcription Factor SCX is a Potential Serum Biomarker of Fibrotic Diseases.

International journal of molecular sciences

PA5-23943 was used in Western Blotting to propose the bHLH transcription factor scleraxis (SCX) could be a candidate biomarker of fibrotic diseases.

Thu Jul 16 00:00:00 UTC 2020

The Influence of Cell Source and Donor Age on the Tenogenic Potential and Chemokine Secretion of Human Mesenchymal Stromal Cells.

Stem cells international

Zarychta-Wiśniewska W,Burdzińska A,Zielniok K,Koblowska M,Gala K,Pędzisz P,Iwanicka-Nowicka R,Fogtman A,Aksamit A,Kulesza A,Zołocińska A,Pączek L

Published figure using SCXA polyclonal antibody (Product # PA5-23943) in Western Blot

Fri Apr 08 00:00:00 UTC 2022

Angiopoietin-like 4 induces a β-catenin-mediated upregulation of ID3 in fibroblasts to reduce scar collagen expression.

Scientific reports

Teo Z,Chan JSK,Chong HC,Sng MK,Choo CC,Phua GZM,Teo DJR,Zhu P,Choong C,Wong MTC,Tan NS

Published figure using SCXA polyclonal antibody (Product # PA5-23943) in Western Blot

Mon Jul 24 00:00:00 UTC 2017

Angiopoietin-like 4 induces a β-catenin-mediated upregulation of ID3 in fibroblasts to reduce scar collagen expression.

Scientific reports

Teo Z,Chan JSK,Chong HC,Sng MK,Choo CC,Phua GZM,Teo DJR,Zhu P,Choong C,Wong MTC,Tan NS

Published figure using SCXA polyclonal antibody (Product # PA5-23943) in Western Blot

Mon Jul 24 00:00:00 UTC 2017

生物信息学

蛋白别名: Basic helix-loop-helix transcription factor scleraxis; bHLHa41; bHLHa48; Class A basic helix-loop-helix protein 41; Class A basic helix-loop-helix protein 48; class II bHLH protein scleraxis; scleraxis basic helix-loop-helix transcription factor; scleraxis homolog A; scleraxis homolog B

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基因别名: BB114693; BHLHA41; BHLHA48; Scl; SCX; SCXA; SCXB

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UniProt ID: (Human) Q7RTU7, (Mouse) Q64124

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Entrez Gene ID: (Human) 642658, (Mouse) 20289

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功能

RNA polymerase II distal enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding DNA binding bHLH transcription factor binding sequence-specific DNA binding protein heterodimerization activity E-box binding protein binding protein dimerization activity

参与通路

mesoderm formation tissue homeostasis endochondral ossification chondrocyte differentiation heart valve morphogenesis heart valve formation transcription, DNA-templated transcription from RNA polymerase II promoter positive regulation of cell proliferation positive regulation of gene expression cell differentiation collagen fibril organization BMP signaling pathway positive regulation of collagen biosynthetic process skeletal muscle cell differentiation tendon development tendon cell differentiation tendon formation deltoid tuberosity development negative regulation of apoptotic process negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter embryonic skeletal system development Sertoli cell differentiation face morphogenesis regulation of cartilage development positive regulation of cartilage development sclerotome development cellular response to mechanical stimulus cellular response to transforming growth factor beta stimulus positive regulation of gastrulation regulation of transcription, DNA-templated multicellular organism development cartilage development

SCXA Polyclonal Antibody

SCXA Antibody (PA5-23943) in IHC (P)

产品信息

PA5-23943

免疫印迹 (WB)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

染色质免疫沉淀实验 (ChIP)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

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SCXA Polyclonal Antibody

SCXA Antibody (PA5-23943) in IHC (P)

产品信息

PA5-23943

免疫印迹 (WB)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

染色质免疫沉淀 实验 (ChIP)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

染色质免疫沉淀实验 (ChIP)