Invitrogen

SDHA Recombinant Rabbit Monoclonal Antibody (JM10-83)

SDHA Antibody in Immunocytochemistry (ICC/IF)

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SDHA Antibody (MA5-32598) in ICC/IF

Immunofluorescence analysis of SDHA was performed using 70 confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with SDHA Recombinant Rabbit Monoclonal Antibody (JM10-83) (Product # MA5-32598) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor ... View More

SDHA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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SDHA Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. SH-SY5Y cells were transfected with SDHA siRNA and decrease in signal intensity was observed in Western Blot application using Anti-SDHA Recombinant Rabbit Monoclonal Antibody (JM10-83) (Product # MA5-32598). {KD}

产品信息

MA5-32598

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

免疫组化（石蜡） (IHC (P))

1:1,000-1:5,000

免疫细胞化学 (ICC/IF)

1:100-1:500

流式细胞分析 (Flow)

1:1,000

产品规格

种属反应

Human, Mouse, Rat, Zebrafish

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JM10-83

抗原

Synthetic peptide within Human SDHA aa 581-624

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2809875

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: Flavoprotein subunit of complex II; Fp; Malate dehydrogenase [quinone] flavoprotein subunit; succinate dehydrogenase [ubiquinone] flavoprotein subunit; Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial; succinate dehydrogenase complex subunit A, flavoprotein (Fp); succinate dehydrogenase complex, subunit A, flavoprotein (Fp)

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基因别名: 1500032O14Rik; 2310034D06Rik; 4921513A11; C81073; CMD1GG; FP; im:7141001; PGL5; SDH1; SDH2; SDHA; SDHF; si:dkeyp-84f11.2; zgc:56051