Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 2
SEMA3E Antibody (PA5-47469) in IHC (F)
Immunohistochemical analysis of SEMA3E in immersion fixed frozen sections of adult mouse brain. Samples were incubated in SEMA3E polyclonal antibody (Product # PA5-47469) using a dilution of 10 µg/mL overnight at 4 °C followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cerebellum.
产品信息
PA5-47469
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Human
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Mouse myeloma cell line NS0-derived recombinant human Semaphorin 3E Thr25-Ser775 (Arg557Ala and Arg560Ala)
偶联物
形式
Lyophilized
浓度
0.2 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 5% trehalose
内含物
No Preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2610392
产品详细信息
In direct ELISAs, approximately 30% cross-reactivity with recombinant mouse (rm) Semaphorin 3E is observed and less than 10% cross-reactivity with rmSemaphorin 3A, rmSemaphorin 3B, rmSemaphorin 3C, rmSemaphorin 3F, and recombinant human (rh) Semaphorin 3A, rhSemaphorin 3C, rhSemaphorin 3D, and rhSemaphorin 3F is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
靶标信息
The Semaphorins make up the largest family of axon guidance cues yet described. Semaphorins are divided into 8 classes (classes 3-7 found in vertebrates). Class 3 Semaphorins are secreted, classes 4 through 6 are transmembrane proteins, and class 7 are membrane associated via glycosylphosphatidylinositol (GPI) linkage. They are characterized structurally by a conserved approximately 400 amino acid sema domain. They are classically described as collapsing factors and mediators of axon repulsion, although they may also act as context-dependent chemoattractants. Semaphorins have been shown to have roles in cardiovascular development and in the regulation of immune cell antigen presentation. Receptors or receptor complexes that mediate semaphorin signaling include proteins of the Neuropilin and Plexin families.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: M-sema H; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E; Sema H; Semaphorin-3E; Semaphorin-H
基因别名: 6430702L12; AA408817; coll-5; KIAA0331; M-SEMAH; M-SemaK; mKIAA0331; SEMA3E; SEMAH; Semh
UniProt ID: (Human) Q75M97, (Mouse) P70275
Entrez Gene ID: (Human) 9723, (Mouse) 20349
patterning of blood vessels
neural crest cell migration
negative regulation of cell-matrix adhesion
sprouting angiogenesis
regulation of cell shape
negative regulation of angiogenesis
positive regulation of cell migration
negative regulation of axon extension involved in axon guidance
synapse organization
negative chemotaxis
semaphorin-plexin signaling pathway
regulation of actin cytoskeleton reorganization
angiogenesis
multicellular organism development
nervous system development
cell differentiation
