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SEMA5B Antibody (PA5-113369) in ICC/IF
Immunocytochemichal analysis of SEMA5B in Hela cells using a Polyclonal antibody (Product # PA5-113369) at a dilution of 1:33. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were also counter-stained with DAPI.
产品信息
PA5-113369
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant Human Semaphorin-5B protein (845-1013AA)
偶联物
形式
Liquid
浓度
1.04 mg/mL
纯化类型
Protein G
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.03% ProClin 300
保存条件
-20°C or -80°C if preferred
运输条件
Wet ice
RRID
AB_2868103
靶标信息
May act as positive axonal guidance cues.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B; Sema G; semaphorin 5B; Semaphorin-5B; Semaphorin-G
基因别名: AI893641; KIAA1445; mKIAA1445; SEMA5B; SEMAG; SemG; UNQ5867/PRO34001
UniProt ID: (Human) Q9P283, (Mouse) Q60519
Entrez Gene ID: (Human) 54437, (Mouse) 20357
neural crest cell migration
positive regulation of cell migration
axon extension
negative regulation of axon extension involved in axon guidance
detection of light stimulus involved in visual perception
negative chemotaxis
semaphorin-plexin signaling pathway
neuron projection guidance
multicellular organism development
nervous system development
cell differentiation
