Invitrogen

SETD7 Polyclonal Antibody

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SETD7 Antibody (PA5-97320) in WB

Western blot analysis of SETD7 in extracts from normal (control) and SETD7 knockout (KO) 293T cells. Samples were incubated with SETD7 Polyclonal antibody (Product # PA5-97320) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Product Image Gallery

SETD7 Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Loss of signal was observed for target protein in SETD7 knockout (KO) HeLa cells cell line using Anti-SETD7 Polyclonal Antibody (Product # PA5-97320). {KO}

产品信息

PA5-97320

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

免疫细胞化学 (ICC/IF)

1:50-1:200

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 1-366 of human SETD7 (NP_0851511)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.48 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2809122

产品详细信息

Immunogen sequence: MDSDDEMVEE AVEGHLDDDG LPHGFCTVTY SSTDRFEGNF VHGEKNGRGK FFFFDGSTLE GYYVDDALQG QGVYTYEDGG VLQGTYVDGE LNGPAQEYDT DGRLIFKGQY KDNIRHGVCW IYYPDGGSLV GEVNEDGEMT GEKIAYVYPD ERTALYGKFI DGEMIEGKLA TLMSTEEGRP HFELMPGNSV YHFDKSTSSC ISTNALLPDP YESERVYVAE SLISSAGEGL FSKVAVGPNT VMSFYNGVRI THQEVDSRDW ALNGNTLSLD EETVIDVPEP YNHVSKYCAS LGHKANHSFT PNCIYDMFVH PRFGPIKCIR TLRAVEADEE LTVAYGYDHS PPGKSGPEAP EWYQVELKAF QATQQK; Positive Samples: HeLa, HepG2, Mouse pancreas, Mouse brain, Rat brain; Cellular Location: Chromosome, Nucleus

靶标信息

Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation. [UniProt]

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: FLJ21193; H3-K4 methyltransferase; H3-K4-HMTase; H3-K4-HMTase SETD7; Histone H3-K4 methyltransferase SETD7; histone H3-lysine 4-specific methyltransferase; Histone-lysine N-methyltransferase SETD7; Lysine N-methyltransferase 7; OTTHUMP00000220049; SET domain containing (lysine methyltransferase) 7; SET domain-containing protein 7; SET7/9

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基因别名: 1600028F23Rik; H3K4MT; KIAA1717; KMT7; mKIAA1717; SET7; SET7/9; SET9; SETD7

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UniProt ID: (Human) Q8WTS6, (Mouse) Q8VHL1

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Entrez Gene ID: (Human) 80854, (Rat) 689954, (Mouse) 73251

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功能