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SF3B14 Antibody (PA5-88980) in WB
Western blot analysis of SF3B14 in extracts of various cell lines. Samples were incubated with SF3B14 Polyclonal antibody (Product # PA5-88980) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
产品信息
PA5-88980
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-125 of human SF3B6 (NP_0571311)
偶联物
形式
Liquid
浓度
2.68 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2805268
产品详细信息
Immunogen sequence: MAMQAAKRAN IRLPPEVNRI LYIRNLPYKI TAEEMYDIFG KYGPIRQIRV GNTPETRGTA YVVYEDIFDA KNACDHLSGF NVCNRYLVVL YYNANRAFQK MDTKKKEEQL KLLKEKYGIN TDPPK; Positive Samples: K-562, HeLa, Mouse spleen; Cellular Location: Nucleus
靶标信息
SF3B14 encodes a 14 kDa protein subunit of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. This 14 kDa protein interacts directly with subunit 1 of the splicing factor 3b complex. This 14 kDa protein also interacts directly with the adenosine that carries out the first transesterification step of splicing at the pre-mRNA branch site.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: pre mRNA branch site protein p14; Pre-mRNA branch site protein p14; SF3B 14 kDa subunit; SF3B14a; spliceosome-associated protein, 14 kDa subunit; Spliceosome-associated protein, 14-kDa; Splicing factor 3B subunit 6; splicing factor 3B, 14 kDa subunit; Splicing factor 3b, subunit 6, 14kDa
基因别名: 0610009D07Rik; 6030419K15Rik; AV001342; CGI-110; HSPC175; HT006; P14; SAP14; SAP14a; SF3B14; SF3B14A; SF3B6
UniProt ID: (Human) Q9Y3B4, (Mouse) P59708
Entrez Gene ID: (Human) 51639, (Mouse) 66055
