Invitrogen

SFTPB Monoclonal Antibody (SPB02 (1B9))

4 Published Figures

5 References

View all (18) SFTPB antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)
Published Figures (4)

SFTPB Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

SFTPB Antibody (MA1-204) in ICC/IF

Immunofluorescent analysis of Surfactant Protein B (green) in A549 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Surfactant Protein B monoclonal antibody (Product # MA1-204) at a dilution of 1:50 in staining buffer for 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG Superclonal™ Secondary Antibody, Alexa Fluor 488 conjugate... View More

SFTPB Antibody in Immunohistochemistry (Frozen) (IHC (F))

SFTPB Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))

SFTPB Antibody in Immunohistochemistry (IHC)

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Product Details

MA1-204

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

1:100

Immunocytochemistry (ICC/IF)

1:50-1:100

Product Specifications

Species Reactivity

Human

Published species

Human, Rhesus monkey

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

SPB02 (1B9)

Immunogen

Recombinant Surfactant Protein B

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2633311

Product Specific Information

Detects mature surfactant protein B (8kDa monomer and 16kDa Dimeric forms) and pro surfactant protein B (24kDa) in western blot.

Sequence homology: Cow: 86%; Dog: 100%; Guinea Pig: 100%; Human: 100%; Mouse: 92%; Rabbit: 85%; Rat: 100%; Sheep: 79%.

Target Information

Pulmonary surfactant is a complex mixture of phospholipids and proteins that is secreted from type II cells in alveoli and reduces the surface tension at the alveolar air-liquid interface, providing alveolar stability necessary for normal ventilation. Four distinct proteins isolated from pulmonary surfactant are termed surfactant proteins A, B, C, and D. SP-A (28-36kDa) and SP-D (43kDa) are collagenous carbohydrate-binding proteins, whereas SP-B (8-9kDa) and SP-C (4kDa) are non-collagenous hydrophobic proteins. SP-B is expressed in pulmonary adenocarcinomas with acinar, papillary, bronchioloalveolar, and solid growth patterns. Squamous cell and large cell carcinomas of the lung and nonpulmonary adenocarcinomas do not express SP-B. ProSP-B is glycosylated in the Golgi apparatus and undergoes carboxy- and amino-terminal proteolysis by a cathepsin D-like protease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Organoid-based expansion of patient-derived primary alveolar type 2 cells for establishment of alveolus epithelial Lung-Chip cultures.

American journal of physiology. Lung cellular and molecular physiology

van Riet S,van Schadewijk A,Khedoe PPSJ,Limpens RWAL,Bárcena M,Stolk J,Hiemstra PS,van der Does AM

Published figure using SFTPB monoclonal antibody (Product # MA1-204) in Immunohistochemistry

Fri Apr 01 00:00:00 UTC 2022

Human distal lung maps and lineage hierarchies reveal a bipotent progenitor.

Nature

Kadur Lakshminarasimha Murthy P,Sontake V,Tata A,Kobayashi Y,Macadlo L,Okuda K,Conchola AS,Nakano S,Gregory S,Miller LA,Spence JR,Engelhardt JF,Boucher RC,Rock JR,Randell SH,Tata PR

MA1-204 was used in Immunocytochemistry, Western Blot, Immunohistochemistry (Paraffin) to revise human lung cell maps and lineage trajectories, and implicate an epithelial transitional state in primate lung regeneration and disease.

Fri Apr 01 00:00:00 UTC 2022

Human Lung Stem Cell-Based Alveolospheres Provide Insights into SARS-CoV-2-Mediated Interferon Responses and Pneumocyte Dysfunction.

Cell stem cell

Katsura H,Sontake V,Tata A,Kobayashi Y,Edwards CE,Heaton BE,Konkimalla A,Asakura T,Mikami Y,Fritch EJ,Lee PJ,Heaton NS,Boucher RC,Randell SH,Baric RS,Tata PR

MA1-204 was used in Immunohistochemistry to report a feeder-free, scalable, chemically defined, and modular alveolosphere culture system for the propagation and differentiation of human alveolar type 2 cells/pneumocytes derived from primary lung tissue.

Thu Dec 03 00:00:00 UTC 2020

Human Lung Stem Cell-Based Alveolospheres Provide Insights into SARS-CoV-2-Mediated Interferon Responses and Pneumocyte Dysfunction.

Cell stem cell

Katsura H,Sontake V,Tata A,Kobayashi Y,Edwards CE,Heaton BE,Konkimalla A,Asakura T,Mikami Y,Fritch EJ,Lee PJ,Heaton NS,Boucher RC,Randell SH,Baric RS,Tata PR

Thu Dec 03 00:00:00 UTC 2020

Human Lung Stem Cell-Based Alveolospheres Provide Insights into SARS-CoV-2-Mediated Interferon Responses and Pneumocyte Dysfunction.

Cell stem cell

Katsura H,Sontake V,Tata A,Kobayashi Y,Edwards CE,Heaton BE,Konkimalla A,Asakura T,Mikami Y,Fritch EJ,Lee PJ,Heaton NS,Boucher RC,Randell SH,Baric RS,Tata PR

Thu Dec 03 00:00:00 UTC 2020

Abnormalities of the TITF-1 lineage-specific oncogene in NSCLC: implications in lung cancer pathogenesis and prognosis.

Clinical cancer research : an official journal of the American Association for Cancer Research

Tang X,Kadara H,Behrens C,Liu DD,Xiao Y,Rice D,Gazdar AF,Fujimoto J,Moran C,Varella-Garcia M,Lee JJ,Hong WK,Wistuba II

MA1-204 was used in Immunohistochemistry to demonstrate that the protein versus genomic patterns of TITF-1 have opposing roles in lung cancer prognosis and may occur preferentially in different subsets of NSCLC patients with distinct oncogene mutations.

Fri Apr 15 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: 18 kDa pulmonary-surfactant protein; 6 kDa protein; P SFTB3; Pulmonary surfactant-associated protein B; Pulmonary surfactant-associated proteolipid SPL(Phe); SFPB; SP-B

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Gene Aliases: PSP-B; SFTB3; SFTP3; SFTPB; SMDP1; SP-B

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UniProt ID: (Human) P07988

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Entrez Gene ID: (Human) 6439

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Function(s)

G-protein coupled receptor binding enzyme activator activity scaffold/adaptor protein

Process(es)

sphingolipid metabolic process respiratory gaseous exchange organ morphogenesis positive regulation of catalytic activity cellular protein metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SFTPB Monoclonal Antibody (SPB02 (1B9))

SFTPB Antibody (MA1-204) in ICC/IF

Product Details

MA1-204

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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