Invitrogen

SHANK1/SHANK3 Monoclonal Antibody (N367/51), FITC

SHANK1/SHANK3 Antibody in Immunocytochemistry (ICC/IF)

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SHANK1/SHANK3 Antibody (MA5-45674) in ICC/IF

Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with SHANK1/SHANK3 monoclonal antibody (Product # MA5-45674) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000; 1:5,00... View More

SHANK1/SHANK3 Antibody in Western Blot (WB)

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产品信息

MA5-45674

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化 (IHC)

Assay-dependent

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

N367/51

抗原

Fusion protein amino acids 538-626 (SH3 domain) of rat SHANK3.

查看免疫原

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

9.1mM sodium bicarbonate/PBS, pH 7.4, with 640.91mM DMSO, 136.36mM ethanolamine

内含物

no preservative

保存条件

4° C, store in dark

运输条件

Ambient (domestic); Wet ice (international)

AB_2932128

产品详细信息

Mouse: 100% identity (89/89 amino acids identical). Human: 97% identity (87/89 amino acids identical). ~70% identity with SHANK1 and SHANK2.

1 µg/mL of MA5-45674 was sufficient for detection of SHANK1/SHANK3 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 190kDa. Cross-reacts with SHANK1. Does not cross-react with SHANK2.

This antibody was formerly sold as clone S367-51.

靶标信息

Major scaffold postsynaptic density protein which interacts with multiple proteins and complexes to orchestrate the dendritic spine and synapse formation, maturation and maintenance. Interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and HOMER, respectively, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction through the interaction with Arp2/3 and WAVE1 complex as well as the promotion of the F-actin clusters. By way of this control of actin dynamics, participates in the regulation of developing neurons growth cone motility and the NMDA receptor-signaling. Also modulates GRIA1 exocytosis and GRM5/MGLUR5 expression and signaling to control the AMPA and metabotropic glutamate receptor-mediated synaptic transmission and plasticity. May be required at an early stage of synapse formation and be inhibited by IGF1 to promote synapse maturation. {PubMed:21606927, PubMed:21795692, PubMed:23100419, PubMed:23897824, PubMed:24089484}.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: GKAP/SAPAP interacting protein; GKAP/SAPAP-interacting protein; proline rich synapse associated protein 2; Proline-rich synapse-associated protein 2; ProSAP2; SAPAP-interacting protein synamon; SH3 and multiple ankyrin repeat domains 1; SH3 and multiple ankyrin repeat domains protein 1; SH3 and multiple ankyrin repeat domains protein 3; SH3 and multiple ankyrin repeat domains protein 3 variant c-3; SH3 and multiple ankyrin repeat domains protein 3 variant c-4; SH3 and multiple ankyrin repeat domains-like protein 3 variant 1; SH3 and multiple ankyrin repeat domains-like protein 3 variant 2; SH3 and multiple ankyrin repeat domains-like protein 3 variant 3; SH3 and multiple ankyrin repeat domains-like protein 3 variant c-1; SH3 and multiple ankyrin repeat domains-like protein 3 variant c-2; SH3 and multiple ankyrin repeat domains-like protein 3 variant d-1; SH3 and multiple ankyrin repeat domains-like protein 3 variant d-2; SH3 and multiple ankyrin repeat domains-like protein 3 variant d-3; SH3 and multiple ankyrin repeat domains-like protein 3 variant e-1; SH3 and multiple ankyrin repeat domains-like protein 3 variant e-2; SH3/ankyrin domain gene 1; SH3/ankyrin domain gene 3; shank postsynaptic density protein; Shank postsynaptic density protein 3a; Shank1; Shank3; shank3 splice variant II; shank3 splice variant III; shank3 splice variant IV; shank3e splice variant III; shank3e splice variant IV; Somatostatin receptor-interacting protein; SPANK-1; SPANK-2; SSTR-interacting protein; Synamon

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基因别名: AI841104; DEL22q13.3; Kiaa1650; PROSAP2; PSAP2; SCZD15; SHANK1; Shank1a; Shank3; Shank3b; SHANK3c-3; SHANK3c-4; SPANK-1; SPANK-2; Spank1; SSTRIP; synamon