Invitrogen

SIAH1/SIAH2 Monoclonal Antibody (8G7H12)

SIAH1/SIAH2 Antibody in Western Blot (WB)

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SIAH1/SIAH2 Antibody (MA5-24781) in WB

Western blot analysis of SIAH1/SIAH2 in drosophila SINA and human SIAH. Samples were incubated in SIAH1/SIAH2 monoclonal antibody (Product # MA5-24781). There are two SINA-like E3 ligases, SINA and SINAH, in Drosophila. SINA monoclonal antibody 8G7H12 is highly specific.

SIAH1/SIAH2 Antibody in RNA Immunoprecipitation (RIP)

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产品信息

MA5-24781

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

免疫沉淀 (IP)

1:10-1:500

RNA免疫共沉淀 (RIP)

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产品规格

种属反应

Fruit fly, Human, Pig, Rat, Zebrafish

已发表种属

Not Applicable

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

8G7H12

抗原

Synthetic peptide made to a C-terminal portion of the drosophila SINA protein (within residues 280-331).

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. Not Determined

保存液

tissue culture supernatant

内含物

0.1% sodium azide

保存条件

4° C, do not freeze

运输条件

Wet ice

AB_2717254

产品详细信息

This is specific for human SIAH 1 and 2 and Drosophila SINA in proliferating cells and stem cells. This does not detect Drosophila SINAH or normal, nondividing cells.

靶标信息

SIAH, the human homologue of the Drosophila seven in absentia (sina) gene, is a tumor suppressor protein that is expressed in intestinal epithelium and activated during apoptosis. Human SIAH proteins are produced as two distinct gene products, SIAH-1, and a slightly larger protein SIAH-2, which share a highly conserved C-terminal sequence and differ in their N-terminal regions. SIAH-1 is a 32 kDa protein that contains an N-terminal RING-finger domain, which is required for proteolysis, and a cystein-rich C-terminal domain, which regulates oligomerization and SIAH binding to target proteins. As a tumor suppressor, SIAH-1 binds DCC (deleted in colorectal cancer) and regulates DCC degradation via the ubiquitin-proteasome pathway. SIAH-1 also binds a Bcl-2 related protein, Bag-1, thereby inhibiting cell growth. The majority of SIAH-1 is localized to the nucleus, however a small percentage is detected in the cytoplasm. This nuclear localization suggests that SIAH proteins may interact with other nuclear matrix proteins and DNA.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Molecular medicine (Cambridge, Mass.)

Hu R,Xu B,Ma J,Li L,Zhang L,Wang L,Zhu J,Guo T,Zhang H,Wang S

Published figure using SIAH1/SIAH2 monoclonal antibody (Product # MA5-24781) in RNA Immunoprecipitation

Thu Jan 05 00:00:00 UTC 2023

生物信息学

蛋白别名: anon-fast-evolving-2H7; CG9949-PA; CG9949-PB; CG9949-PC; E3 ubiquitin-protein ligase SIAH1; E3 ubiquitin-protein ligase SIAH2; E3 ubiquitin-protein ligase sina; EC 6.3.2; FLJ08065; HL08111p; hSIAH1; RING-type E3 ubiquitin transferase SIAH1; RING-type E3 ubiquitin transferase SIAH2; RING-type E3 ubiquitin transferase sina; seven in absenti; seven in absentia 1A; seven in absentia 2; Seven in absentia homolog 1; Seven in absentia homolog 2; Seven in absentia protein; seven-in-absentia; Siah-1; Siah-1a; Siah-2; sina-PA; sina-PB; sina-PC

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基因别名: anon-EST:fe2H7; CG9949; Dmel\CG9949; Dmel_CG9949; hSiah2; HUMSIAH; id:ibd2635; sia; SIAH; SIAH1; SIAH1A; SIAH2; SINA; zgc:56026