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SIK3 Antibody (PA5-115813) in ICC/IF
Immunocytochemistry analysis of SIK3 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115813) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
产品信息
PA5-115813
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human SIK3(Accession Q9Y2K2), corresponding to amino acid residues P586-K636.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2900447
产品详细信息
Antibody detects endogenous levels of total QSK.
靶标信息
SIK3 (QSK) is a serine/threonine-protein kinase, belongs to QIK subfamily. The phosphorylation of SIK3 by LKB1 through the 14-3-3 binding enhances its catalytic activity and leads its localization to punctate structures within the cytoplasm. Overexpression of SIK3 promotes G1/S cell cycle progression with ovarian cancer. There are two sites (H331L and A1103V) were mutated at significant frequency in breast cancer. SIK3 is a novel tumor-associated antigen (TAA). SIK3 is a positive regulator of mTOR signaling that functions by triggering the degradation of DEPTOR, an mTOR inhibitor. Involved in the dynamic regulation of mTOR signaling in chondrocyte differentiation during skeletogenesis. Negatively regulates cAMP signaling pathway possibly by acting on CRTC2/TORC2 and CRTC3/TORC3.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Salt-inducible kinase 3; Serine/threonine-protein kinase QSK; Serine/threonine-protein kinase SIK3; SIK-3
基因别名: 5730525O22Rik; 9030204A07Rik; AI447252; AI846254; AI849445; KIAA0999; L19; mKIAA0999; QSK; SIK-3; SIK3
UniProt ID: (Human) H0Y494, (Mouse) Q6P4S6
Entrez Gene ID: (Human) 23387, (Mouse) 70661, (Rat) 684112
