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Detection of human SIL by western blot of immunoprecipitates.Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SIL antibody A302-441A (lot A302-441A-2) used for IP at 6 µg per reaction. SIL was also immunoprecipitated by rabbit anti-SIL antibody A302-442A. For blotting immunoprecipitated SIL, A302-441A was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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产品信息
A302-441A
应用
建议稀释比
已发表文章
免疫印迹
(WB)
1:10,000-1:25,000
-
免疫沉淀
(IP)
2-10 µg/mg lysate
-
产品规格
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Region between residue 450 and 500 of Human SCL/TAL1 interrupting locus.