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Detection of human SIL by western blot.Samples: Whole cell lysate (15 and 50 µg) from Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SIL antibody A302-441A-M (lot A302-441A-M-2) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.
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