Bethyl Laboratories

SIL Polyclonal Antibody

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抗体检测数据 (2)

SIL Antibody in Western Blot (WB)

SIL Antibody in Western Blot (WB)

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SIL Antibody (A302-442A) in WB

Detection of mouse SIL by western blot. Samples: Whole cell lysate (50 µg) from TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-SIL antibody (Product # A302-442A) (lot A302-442A-1) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

SIL Antibody in Western Blot (WB)

SIL Antibody in Western Blot (WB)

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SIL Antibody in Western Blot (WB) — Detection of mouse SIL by western blot. Samples: Whole cell lysate (50 µg) from TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-SIL antibody (Product # A302-442A) (lot A302-442A-1) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

SIL Antibody in Western Blot (WB) — Detection of human SIL by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SIL antibody (Product # A302-442A) used for WB at 0.04 µg/mL (A) and 1 µg/mL (B) and used for IP at 3 µg/mg lysate. SIL was also immunoprecipitated by rabbit anti-SIL antibody A302-441A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).

产品信息

A302-442A

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000-1:10,000

-

免疫沉淀 (IP)

2-5 µg/mg lysate

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Region between residue 1237 and 1287 of Human SCL/TAL1 interrupting locus.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.20 mg/mL

纯化类型

Antigen affinity chromatography

保存液

TBS, pH 7.0 to 8.0, with 0.1% BSA

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Wet ice

产品详细信息

The recommended shelf life for this product is 1 year from date of receipt.

靶标信息

Plays an essential role in early embryonic development.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: SCL-interrupting locus protein; SCL-interrupting locus protein homolog; TAL-1-interrupting locus protein; Tal1 interrupting locus

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基因别名: MCPH7; SIL; STIL

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UniProt ID: (Human) Q15468, (Mouse) Q60988

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Entrez Gene ID: (Human) 6491, (Mouse) 20460

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protein binding

embryonic axis specification in utero embryonic development neural tube closure heart looping mitotic spindle organization smoothened signaling pathway multicellular organism development determination of left/right symmetry neural tube development forebrain development notochord development floor plate development multicellular organism growth negative regulation of apoptotic process positive regulation of cyclin-dependent protein serine/threonine kinase activity centrosome duplication cilium morphogenesis mitotic spindle checkpoint protein localization to centrosome cell proliferation regulation of centriole replication