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SIL Antibody (PA5-115974) in ICC/IF
Immunocytochemistry analysis of SIL in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115974) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
产品信息
PA5-115974
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human STIL(Accession Q15468), corresponding to amino acid residues E1236-L1286.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2900608
产品详细信息
Antibody detects endogenous levels of total STIL.
靶标信息
Plays an essential role in early embryonic development.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: SCL-interrupting locus protein; SCL-interrupting locus protein homolog; Scl/Tal1 interrupting locus; TAL-1-interrupting locus protein; Tal1 interrupting locus
基因别名: MCPH7; SIL; STIL
UniProt ID: (Human) Q15468, (Mouse) Q60988
Entrez Gene ID: (Human) 6491, (Mouse) 20460, (Rat) 313506
embryonic axis specification
in utero embryonic development
neural tube closure
heart looping
mitotic spindle organization
smoothened signaling pathway
multicellular organism development
determination of left/right symmetry
neural tube development
forebrain development
notochord development
floor plate development
multicellular organism growth
negative regulation of apoptotic process
positive regulation of cyclin-dependent protein serine/threonine kinase activity
centrosome duplication
cilium morphogenesis
mitotic spindle checkpoint
protein localization to centrosome
cell proliferation
regulation of centriole replication
biological_process
