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SIRT3 Antibody (PA5-142763) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of SIRT3 in NIH3T3 cells using SIRT3 Polyclonal Antibody (Product # PA5-142763). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
产品信息
PA5-142763
产品规格
种属反应
Mouse
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Peptide with sequence C-RASGIPASKLVEAH.
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Ammonium sulfate precipitation
保存液
TBS, pH 7.3, with 0.5% BSA
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2933409
产品详细信息
Additional Information: This antibody is expected to recognize both reported isoforms (NP_071878.2; NP_001171275.1).
This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:64,000.
靶标信息
SIRT3 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The SIRT3 is included in class I of the sirtuin family.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: HGNC:14931; mitochondrial protein lysine deacetylase; mSIR2L3; NAD-dependent deacetylase sirtuin-3; NAD-dependent protein deacetylase sirtuin-3; Regulatory protein SIR2 homolog 3; silent mating type information regulation 2, (S.cerevisiae, homolog)-like 3; SIR2-like protein 3; SIRT3L mitochondrial
基因别名: 2310003L23Rik; AI848213; Sir2l3; Sirt3
UniProt ID: (Mouse) Q8R104
Entrez Gene ID: (Mouse) 64384
histone deacetylase activity
protein binding
zinc ion binding
protein-N-terminal asparagine amidohydrolase activity
UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase activity
hydrolase activity
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
iprodione amidohydrolase activity
(3,5-dichlorophenylurea)acetate amidohydrolase activity
enzyme binding
NAD-dependent histone deacetylase activity (H3-K14 specific)
4'-(2-hydroxyisopropyl)phenylurea amidohydrolase activity
didemethylisoproturon amidohydrolase activity
N-isopropylacetanilide amidohydrolase activity
N-cyclohexylformamide amidohydrolase activity
isonicotinic acid hydrazide hydrolase activity
cis-aconitamide amidase activity
gamma-N-formylaminovinylacetate hydrolase activity
N2-acetyl-L-lysine deacetylase activity
O-succinylbenzoate synthase activity
indoleacetamide hydrolase activity
N-acetylcitrulline deacetylase activity
metal ion binding
N-acetylgalactosamine-6-phosphate deacetylase activity
NAD binding
diacetylchitobiose deacetylase activity
chitooligosaccharide deacetylase activity
NAD+ binding
