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Invitrogen
SIRT4 Polyclonal Antibody
FIGURE: 1 / 10
SIRT4 Antibody (PA5-114377) in IHC (P)
Immunohistochemistry analysis of SIRT4 in paraffin-embedded human gastric cancer tissue. Antigen retrieval was performed with citrate buffer (pH6, 20 min). Samples were blocked with 10% goat serum and incubated in SIRT4 polyclonal antibody (Product # PA5-114377) at a dilution of 1 µg/mL (overnight, 4°C), followed by biotinylated goat anti-rabbit IgG (30 min, 37°C) and Strepavidin-Biotin-Complex (SABC) with ... View More
Product Details
PA5-114377
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence of human SIRT4 (HLKPDVVFFGDTVNPDKVDFVHKRVKEAD).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2884833
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat brain tissue, rat heart tissue, rat liver tissue, mouse brain tissue, mouse heart tissue, mouse liver tissue. IHC: human ovary cancer tissue, human placenta tissue, human gastric cancer tissue, human glioma tissue, human rectal cancer tissue, human tonsil tissue.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: MGC130046; MGC130047; MGC57437; NAD-dependent ADP-ribosyltransferase sirtuin-4; NAD-dependent protein biotinylase sirtuin-4; NAD-dependent protein deacetylase sirtuin-4; NAD-dependent protein lipoamidase sirtuin-4, mitochondrial; Regulatory protein SIR2 homolog 4; sir2-like 4; SIR2-like protein 4; SIRT4; sirtuin type 4
Gene Aliases: 4930596O17Rik; SIR2L4; SIRT4
UniProt ID: (Human) Q9Y6E7, (Mouse) Q8R216
Entrez Gene ID: (Human) 23409, (Mouse) 75387, (Rat) 304539
NAD+ ADP-ribosyltransferase activity
protein binding
zinc ion binding
NAD-dependent protein deacetylase activity
biotinidase activity
lipoamidase activity
NAD+ binding
imidazoleglycerol-phosphate synthase activity
histone deacetylase activity
protein-N-terminal asparagine amidohydrolase activity
UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase activity
transferase activity
hydrolase activity
NAD(P)-cysteine ADP-ribosyltransferase activity
NAD(P)-asparagine ADP-ribosyltransferase activity
NAD(P)-serine ADP-ribosyltransferase activity
iprodione amidohydrolase activity
(3,5-dichlorophenylurea)acetate amidohydrolase activity
4'-(2-hydroxyisopropyl)phenylurea amidohydrolase activity
didemethylisoproturon amidohydrolase activity
N-isopropylacetanilide amidohydrolase activity
N-cyclohexylformamide amidohydrolase activity
isonicotinic acid hydrazide hydrolase activity
cis-aconitamide amidase activity
gamma-N-formylaminovinylacetate hydrolase activity
N2-acetyl-L-lysine deacetylase activity
O-succinylbenzoate synthase activity
indoleacetamide hydrolase activity
7-cyano-7-deazaguanine tRNA-ribosyltransferase activity
N-acetylcitrulline deacetylase activity
purine deoxyribosyltransferase activity
metal ion binding
N-acetylgalactosamine-6-phosphate deacetylase activity
diacetylchitobiose deacetylase activity
chitooligosaccharide deacetylase activity
molecular_function
protein ADP-ribosylation
glutamine metabolic process
cellular response to DNA damage stimulus
negative regulation of cardiac muscle cell apoptotic process
peptidyl-lysine deacetylation
negative regulation of fatty acid oxidation
negative regulation of insulin secretion
positive regulation of lipid biosynthetic process
tricarboxylic acid metabolic process
negative regulation of protein processing involved in protein targeting to mitochondrion
regulation of glutamine family amino acid metabolic process
chromatin silencing
cellular response to hypoxia
regulation of pyruvate dehydrogenase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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