Invitrogen

SIX2 Polyclonal Antibody

查看其他7个SIX2抗体

抗体检测数据 (2)

SIX2 Antibody in Western Blot (WB)

SIX2 Antibody in Western Blot (WB)

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SIX2 Antibody (PA5-97149) in WB

Western blot analysis of SIX2 in extracts of various cell lines. Samples were incubated with SIX2 Polyclonal antibody (Product # PA5-97149) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

SIX2 Antibody in Western Blot (WB)

SIX2 Antibody in Western Blot (WB)

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SIX2 Antibody in Western Blot (WB) — Western blot analysis of SIX2 in extracts of various cell lines. Samples were incubated with SIX2 Polyclonal antibody (Product # PA5-97149) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

SIX2 Antibody in Western Blot (WB) — Western blot analysis of SIX2 in extracts of Rat skeletal muscle cells. Samples were incubated with SIX2 Polyclonal antibody (Product # PA5-97149) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

产品信息

PA5-97149

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

-

酶联免疫吸附实验 (ELISA)

1 µg/mL

-

产品规格

种属反应

Human, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 120-174 of human SIX2 (NP_0586283)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.4 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.01% thimerosal

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2808951

产品详细信息

Immunogen sequence: SIWDGEETSY CFKEKSRSVL REWYAHNPYP SPREKRELAE ATGLTTTQVS NWFKN

Positive Samples: Rat kidney; Cellular Location: Nucleus

靶标信息

This gene is a member of the vertebrate gene family which encode proteins homologous to the Drosophila 'sine oculis' homeobox protein. SIX2 is a transcription factor which, like other members of this gene family, may be involved in limb or eye development.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Homeobox protein SIX2; Sine oculis homeobox homolog 2; sine oculis-related homeobox 2 homolog

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基因别名: SIX2

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UniProt ID: (Human) Q9NPC8

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Entrez Gene ID: (Human) 10736, (Rat) 366542

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RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, sequence-specific DNA binding transcription factor binding protein complex binding sequence-specific DNA binding homeodomain transcription factor

kidney development chondrocyte differentiation mesenchymal to epithelial transition involved in metanephros morphogenesis transcription from RNA polymerase II promoter protein import into nucleus mesodermal cell fate specification cell proliferation anatomical structure morphogenesis anterior/posterior axis specification cell migration regulation of ossification regulation of chondrocyte differentiation middle ear morphogenesis negative regulation of cell differentiation positive regulation of transcription from RNA polymerase II promoter embryonic digestive tract morphogenesis embryonic cranial skeleton morphogenesis nephron development nephron morphogenesis mesenchymal stem cell maintenance involved in nephron morphogenesis mesenchymal cell differentiation involved in kidney development regulation of branching involved in ureteric bud morphogenesis mesenchymal stem cell proliferation positive regulation of chondrocyte proliferation metanephros development regulation of transcription, DNA-templated embryonic skeletal system morphogenesis