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SLC5A7 Antibody (PA5-143934) in WB
Western blot analysis of SLC5A7 using SLC5A7 antibody (Product # PA5-143934). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: human A549 whole cell lysates; Lane 2: human RT4 whole cell lysates; Lane 3: rat C6 whole cell lysates; Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Block... View More
Product Details
PA5-143934
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human SLC5A7 recombinant protein (Position: R446-Q580).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_3075148
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human A549 whole cell, humna RT4 whle cell, rat C6 whole cell, mouse NIH/3T3 whole cell. Flow: JK cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Acetylcholine functions as a neurotransmitter in both the central and peripheral nervous systems of all vertebates, and is the principle neurotransmitter used at the neuromuscular junction. Presynaptic synthesis of acetylcholine requires a steady supply of choline, which is acquired by a plasma membrane high affinity choline transporter (CHT).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CHT; hCHT; hCHT1; Hemicholinium-3-sensitive choline transporter; High affinity choline transporter 1; high affinity choline transporter; hemicholinium-3-sensitive choline transporter; MGC126299; MGC126300; rCHT1; solute carrier family 5 (choline transporter), member 7; solute carrier family 5 (sodium/choline cotransporter), member 7; Solute carrier family 5 member 7; solute carrier family 5, member 7
Gene Aliases: CHT; CHT1; HMN7A; SLC5A7
UniProt ID: (Human) Q9GZV3, (Mouse) Q8BGY9, (Rat) Q9JMD7
Entrez Gene ID: (Human) 60482, (Mouse) 63993, (Rat) 85426
transport
ion transport
sodium ion transport
synaptic transmission, cholinergic
neuromuscular synaptic transmission
acetylcholine biosynthetic process
organic cation transport
choline transport
sodium ion transmembrane transport
neurotransmitter biosynthetic process
transmembrane transport
ammonium transmembrane transport
neurotransmitter secretion
cation transmembrane transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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