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SMAD3 Polyclonal Antibody

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说明书
实验方案
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产品信息

600-401-973

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:500-1:10,000
-

酶联免疫吸附实验 (ELISA)

1:15,000-1:50,000
-
产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Smad3 protein.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.06 mg/mL

纯化类型

Affinity chromatography

保存液

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

内含物

0.01% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Directed against human Smad3 protein. A BLAST analysis was used to suggest cross-reactivity with Smad3 protein from human, mouse and rat based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known. Also, the antibody is Smad3 specific, and reactivity to other Smad proteins (specifically Smad1, Smad2, Smad4, and Smad7) is not detected in over-expressed cell lysates (Personal Communication, Kathleen Flanders, CCR-NCI, Bethesda, MD).

靶标信息

SMAD3 belongs to the SMAD family of proteins. They are similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: DKFZp586N0721; DKFZp686J10186; hMAD-3; hSMAD3; JV15-2; MAD (mothers against decapentaplegic, Drosophila) homolog 3; MAD homolog 3; mad homolog JV15-2; mad protein homolog; MAD, mothers against decapentaplegic homolog 3; MAD-3; mad3; MGC60396; mMad3; Mothers against decapentaplegic homolog 3; mothers against DPP homolog 3; SMA- and MAD-related protein 3; SMAD; SMAD 3; SMAD family member 3; SMAD, mothers against DPP homolog 3

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基因别名: AU022421; HSPC193; HsT17436; JV15-2; LDS1C; LDS3; mad3; MADH3; Smad 3; SMAD3

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UniProt ID: (Human) P84022, (Mouse) Q8BUN5, (Rat) P84025

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Entrez Gene ID: (Human) 4088, (Mouse) 17127, (Rat) 25631

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功能
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter sequence-specific core promoter proximal region sequence-specific DNA binding transcription factor activity, protein binding RNA polymerase II activating transcription factor binding transcription factor activity, sequence-specific DNA binding transforming growth factor beta receptor binding protein binding collagen binding transcription factor binding zinc ion binding protein kinase binding phosphatase binding transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity chromatin DNA binding ubiquitin protein ligase binding enhancer binding identical protein binding protein homodimerization activity ubiquitin binding bHLH transcription factor binding sequence-specific DNA binding transcription regulatory region DNA binding protein heterodimerization activity co-SMAD binding R-SMAD binding DNA binding chromatin binding double-stranded DNA binding beta-catenin binding enzyme binding SMAD binding metal ion binding DNA-binding transcription factor gene-specific transcriptional regulator
参与通路
negative regulation of transcription from RNA polymerase II promoter ureteric bud development response to hypoxia in utero embryonic development mesoderm formation somitogenesis liver development heart looping osteoblast development immune system development transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transport activation of cysteine-type endopeptidase activity involved in apoptotic process immune response cell cycle arrest transforming growth factor beta receptor signaling pathway SMAD protein complex assembly endoderm development embryonic pattern specification positive regulation of gene expression positive regulation of alkaline phosphatase activity positive regulation of epithelial to mesenchymal transition regulation of striated muscle tissue development regulation of transforming growth factor beta receptor signaling pathway evasion or tolerance of host defenses by virus signal transduction involved in regulation of gene expression negative regulation of cell growth positive regulation of cell migration positive regulation of bone mineralization negative regulation of transforming growth factor beta receptor signaling pathway thyroid gland development primary miRNA processing positive regulation of chondrocyte differentiation positive regulation of interleukin-1 beta production regulation of transforming growth factor beta2 production positive regulation of transforming growth factor beta3 production activin receptor signaling pathway negative regulation of osteoblast proliferation nodal signaling pathway wound healing T cell activation positive regulation of transcription factor import into nucleus negative regulation of apoptotic process cell-cell junction organization negative regulation of fat cell differentiation negative regulation of osteoblast differentiation positive regulation of transcription, DNA-templated negative regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter paraxial mesoderm morphogenesis developmental growth embryonic foregut morphogenesis embryonic cranial skeleton morphogenesis regulation of epithelial cell proliferation negative regulation of inflammatory response regulation of immune response positive regulation of positive chemotaxis regulation of binding positive regulation of stress fiber assembly positive regulation of focal adhesion assembly pericardium development transdifferentiation SMAD protein signal transduction negative regulation of wound healing lens fiber cell differentiation extrinsic apoptotic signaling pathway activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway positive regulation of extracellular matrix assembly skeletal system development osteoblast differentiation negative regulation of protein phosphorylation regulation of transcription, DNA-templated transcription from RNA polymerase II promoter gastrulation negative regulation of cell proliferation positive regulation of catenin import into nucleus intracellular signal transduction negative regulation of protein catabolic process protein stabilization positive regulation of canonical Wnt signaling pathway
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