Invitrogen

SMC1 Recombinant Rabbit Monoclonal Antibody (SN20-27)

SMC1 Antibody in Immunocytochemistry (ICC/IF)

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SMC1 Antibody (MA5-32341) in ICC/IF

Immunofluorescence analysis of SMC1A/SMC1B was performed using 70% confluent log phase U-2 OS cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SMC1 Recombinant Rabbit Monoclonal Antibody (SN20-27) (Product # MA5-32341) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor... View More

SMC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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SMC1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. MOLT-4 cells were transfected with SMC1A/SMC1B siRNA and decrease in signal intensity was observed in Western Blot application using Anti-SMC1 Recombinant Rabbit Monoclonal Antibody (SN20-27) (Product # MA5-32341). {KD}

产品信息

MA5-32341

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

免疫组化（石蜡） (IHC (P))

1:200-1:1,000

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

SN20-27

抗原

Synthetic peptide within Human SMC1A aa 1,184-1,233

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2809622

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Structural Maintenance of Chromosomes (SMC) family proteins play critical roles in various nuclear events that require structural changes of chromosomes, including mitotic chromosome organization, DNA recombination and repair and global transcriptional repression. The chromo proteins are conserved in eukaryotes and lead to mitotic chromosome segregatior defects. This suggests a critical function of SMC family proteins in mitotic chromosome dynamics. SMC1 and SMC3 form a heterodimeric complex required for metaphase progression in mitotic cells. Specifically this SMC1/SMC3 complex is responsible for sister chromatid cohesion during metaphase. Mutations in the gene can lead to Cornelia de Lange syndrome 2.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: Chromosome segregation protein SmcB; DKFZp686L19178; DXhXs423e; MGC138332; mitosis-specific chromosome segregation protein like protein beta; RP6-29D12.1; Sb1.8; segregation of mitotic chromosomes 1; segregation of mitotic chromosomes b; segregation of mitotic chromosomes-like 1; SM1A; SMC; SMC (segregation of mitotic chromosomes 1)-like 1; SMC protein 1A; SMC protein 1B; SMC-1-alpha; SMC-1-beta; SMC-like 1; SMC-protein; SMC1 (structural maintenance of chromosomes 1, yeast)-like 1; SMC1 structural maintenance of chromosomes 1-like 2; SMC1alpha protein; structural maintenace of chromosomes 1B; structural maintenance of chromosomes 1 like 1; Structural maintenance of chromosomes protein 1A; Structural maintenance of chromosomes protein 1B

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基因别名: 5830426I24Rik; CDLS2; DXS423E; KIAA0178; mKIAA0178; SB1.8; SMC-1A; SMC-1B; SMC1; SMC1A; SMC1alpha; SMC1B; SMC1BETA; SMC1L1; SMC1L2; SMCB