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SMC5 Antibody (PA5-115931) in ICC/IF
Immunocytochemistry analysis of SMC5 in HepG2 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115931). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.
产品信息
PA5-115931
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human SMC5(Accession Q8IY18), corresponding to amino acid residues E964-P1014.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2900565
产品详细信息
Antibody detects endogenous levels of total SMC5.
靶标信息
Core component of the SMC5-SMC6 complex, a complex involved in repair of DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Required for recruitment of telomeres to PML nuclear bodies. Required for sister chromatid cohesion during prometaphase and mitotic progression; the function seems to be independent of SMC6. SMC5-SMC6 complex may prevent transcription of episomal DNA, such as circular viral DNA genome.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: hSMC5; MLZ-453; Protein expressed in male leptotene and zygotene spermatocytes 453; SMC protein 5; SMC-5; SMC5 structural maintenance of chromosomes 5-like 1; Structural maintenance of chromosomes protein 5
基因别名: KIAA0594; mKIAA0594; MLZ-453; mSMC5; SMC-5; SMC5; SMC5L1
UniProt ID: (Human) Q8IY18, (Mouse) Q8CG46
Entrez Gene ID: (Human) 23137, (Mouse) 226026, (Rat) 293967
telomere maintenance via recombination
double-strand break repair via homologous recombination
double-strand break repair via nonhomologous end joining
cellular response to DNA damage stimulus
sister chromatid cohesion
mitotic nuclear division
protein sumoylation
positive regulation of maintenance of mitotic sister chromatid cohesion
cell division
positive regulation of chromosome segregation
resolution of recombination intermediates
cellular senescence
DNA repair
DNA recombination
cell cycle
positive regulation of mitotic metaphase/anaphase transition
