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SMC5 Antibody (PA5-30460) in WB
Western Blot using SMC5 Polyclonal Antibody (Product # PA5-30460). Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with SMC5 Polyclonal Antibody (Product # PA5-30460) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Product Details
PA5-30460
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Zebrafish
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 257 and 493 of Human SMC5
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2547934
Product Specific Information
Recommended positive controls: NT2D1.
Predicted reactivity: Mouse (80%), Rat (82%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Core component of the SMC5-SMC6 complex, a complex involved in repair of DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Required for recruitment of telomeres to PML nuclear bodies. Required for sister chromatid cohesion during prometaphase and mitotic progression; the function seems to be independent of SMC6. SMC5-SMC6 complex may prevent transcription of episomal DNA, such as circular viral DNA genome.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: hSMC5; MLZ-453; Protein expressed in male leptotene and zygotene spermatocytes 453; SMC protein 5; SMC-5; SMC5 structural maintenance of chromosomes 5-like 1; Structural maintenance of chromosomes protein 5
Gene Aliases: KIAA0594; mKIAA0594; MLZ-453; mSMC5; SMC-5; SMC5; SMC5L1
UniProt ID: (Human) Q8IY18, (Mouse) Q8CG46
Entrez Gene ID: (Human) 23137, (Mouse) 226026
telomere maintenance via recombination
double-strand break repair via homologous recombination
double-strand break repair via nonhomologous end joining
cellular response to DNA damage stimulus
sister chromatid cohesion
mitotic nuclear division
protein sumoylation
positive regulation of maintenance of mitotic sister chromatid cohesion
cell division
positive regulation of chromosome segregation
resolution of recombination intermediates
cellular senescence
DNA repair
DNA recombination
cell cycle
positive regulation of mitotic metaphase/anaphase transition
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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