Invitrogen

SMPD3 Polyclonal Antibody

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SMPD3 Antibody (PA5-49140) in WB

Western blot analysis of SMPD3 in HepG2 cell line lysates. Samples were incubated with SMPD3 polyclonal antibody (Product # PA5-49140). Lysates: 35 µg/lane. SMPD3 protein (arrow).

Product Image Gallery

SMPD3 Antibody — FIGURE 2 nSMase2 inhibition was achieved by nSMase2 siRNA and KO in donor cells. (A-C) Western blot analysis of nSMase2 under (A) normoxia and (B) hypoxia with (C) bar graphs showing its expression relative to ß-actin expression (n = 3). (D) nSMase enzymatic activity was determined by a colorimetric assay kit using cell lysates (n = 5). (E) Cytotoxicity of nSMase2 KO and siRNA in cells treated with a-syn under normoxia or hypoxia, as determined by LDH activity (n = 6). The data are presented as the mean ± SEM. *p = 0.05, **p = 0.01. {KD}

产品信息

PA5-49140

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫细胞化学 (ICC/IF)

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 137-165 amino acids from the N-terminal region of human SMPD3.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2 mg/mL

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2634596

产品详细信息

Predicted to react with mouse and rat based on sequence homology.

靶标信息

Catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. Regulates the cell cycle by acting as a growth suppressor in confluent cells. Probably acts as a regulator of postnatal development and participates in bone and dentin mineralization.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Inhibition of nSMase2 Reduces the Transfer of Oligomeric α-Synuclein Irrespective of Hypoxia.

Frontiers in molecular neuroscience

Sackmann V,Sinha MS,Sackmann C,Civitelli L,Bergström J,Ansell-Schultz A,Hallbeck M

PA5-49140 was used in Immunocytochemistry to show that altering sphinolipids can mitigate the spread of α-syn, even under hypoxic conditions, potentially suppressing Parkinson''s disease progression.

Wed Sep 30 00:00:00 UTC 2020

Inhibition of nSMase2 Reduces the Transfer of Oligomeric α-Synuclein Irrespective of Hypoxia.

Frontiers in molecular neuroscience

Sackmann V,Sinha MS,Sackmann C,Civitelli L,Bergström J,Ansell-Schultz A,Hallbeck M

Wed Sep 30 00:00:00 UTC 2020

生物信息学

蛋白别名: Neutral sphingomyelinase 2; Neutral sphingomyelinase II; nSMase-2; Sphingomyelin phosphodiesterase 3; sphingomyelin phosphodiesterase 3, neutral membrane (neutral sphingomyelinase II)

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基因别名: NSMASE2; SMPD3

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UniProt ID: (Human) Q9NY59

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Entrez Gene ID: (Human) 55512

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功能

sphingomyelin phosphodiesterase activity metal ion binding

参与通路

hematopoietic progenitor cell differentiation sphingomyelin catabolic process glycosphingolipid metabolic process cell cycle peptide hormone secretion positive regulation of exosomal secretion positive regulation of ceramide biosynthetic process