Invitrogen

SMYD3 Polyclonal Antibody

SMYD3 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 12

SMYD3 Antibody (PA5-31919) in ICC/IF

SMYD3 Polyclonal Antibody detects SMYD3 protein at cytoplasm by immunofluorescent analysis. Sample: HCT116 cells were fixed in ice-cold MeOH for 5 min. Green: SMYD3 protein stained by SMYD3 Polyclonal Antibody (Product # PA5-31919) diluted at 1:500. Blue: Hoechst 33342 staining.

SMYD3 Antibody in Immunoprecipitation (IP)

Product Image Gallery

SMYD3 Antibody — Fig 5 Gene ablation techniques show no dependence on SMYD2 or SMYD3 for cancer cell proliferation. Waterfall plot representing LogP RSA scores for sgRNAs targeting A) SMYD2 and B) SMYD3. 313 cell lines were infected with a library of 6500 sgRNAs targeting 600 different genes. LogP RSA scores represent depletion of guides from an infected cell population. Each bar represents a different cell line. Bars are colored by cancer subtype. C) Percent confluency of Hep3B cells infected with CRISPR viruses containing CAS9 and sgRNAs targeting HBE-1, EZH2 (negative controls) or SMYD3. Cell density was evaluated using an Incucyte Zoom. Growth curves were initiated 24 days following virus infection and puromycin selection. Plotted data is the average of three biological replicates. Error bars represent standard deviation (not readily visible on scale). D) SMYD3 western blot of lysates derived from Hep3B cells infected with CAS9 and SMYD3 sgRNA. Parental Hep3Bs and Hep3Bs stably infected with HBE-1, EZH2 (negative controls) or SMYD3 were lysed and probed for SMYD3 levels by western. GAPDH levels were evaluated as a loading control. {KO}

产品信息

PA5-31919

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

查看1篇已发表文章 1篇已发表文章

免疫组化 (IHC)

- -

免疫细胞化学 (ICC/IF)

1:100-1:1,000

免疫沉淀 (IP)

1:100-1:500

染色质免疫沉淀实验 (ChIP)

Assay-dependent

产品规格

种属反应

Human, Mouse

已发表种属

Not Applicable

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein fragment corresponding to a region within amino acids 22 and 411 of Human SMYD3

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.23 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2549392

产品详细信息

Recommended positive controls: DDDDK-tagged SMYD3-transfected 293T, 293T, A431, HeLa, HepG2, A549, SKOV3, HCT116.

Predicted reactivity: Human (99%), Mouse (93%), Rat (94%), Pig (95%), Rhesus Monkey (99%), Bovine (83%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

SMYD3 (histone-lysine N-methyltransferase) methylates Lys-4 of histone H3 and Lys-5 of histone H4. It induces di- and tri-methylation. SMYD3 plays an important role in transcriptional activation as a member of an RNA polymerase complex.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Small molecule inhibitors and CRISPR/Cas9 mutagenesis demonstrate that SMYD2 and SMYD3 activity are dispensable for autonomous cancer cell proliferation.

PloS one

Thomenius MJ,Totman J,Harvey D,Mitchell LH,Riera TV,Cosmopoulos K,Grassian AR,Klaus C,Foley M,Admirand EA,Jahic H,Majer C,Wigle T,Jacques SL,Gureasko J,Brach D,Lingaraj T,West K,Smith S,Rioux N,Waters NJ,Tang C,Raimondi A,Munchhof M,Mills JE,Ribich S,Porter Scott M,Kuntz KW,Janzen WP,Moyer M,Smith JJ,Chesworth R,Copeland RA,Boriack-Sjodin PA

Published figure using SMYD3 polyclonal antibody (Product # PA5-31919) in Western Blot

Tue Dec 11 00:00:00 UTC 2018

生物信息学

蛋白别名: bA74P14.1 (novel protein); Histone-lysine N-methyltransferase SMYD3; SET and MYND domain-containing protein 3; Zinc finger MYND domain-containing protein 1; zinc finger protein, subfamily 3A (MYND domain containing), 1; zinc finger, MYND domain containing 1

查看更多收起

基因别名: 2410008A19Rik; bA74P14.1; KMT3E; SMYD3; ZMYND1; ZNFN3A1

查看更多收起

UniProt ID: (Human) Q9H7B4, (Mouse) Q9CWR2

查看更多收起

Entrez Gene ID: (Human) 64754, (Mouse) 69726

查看更多收起

功能

RNA polymerase II core promoter sequence-specific DNA binding RNA polymerase II core binding RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding histone-lysine N-methyltransferase activity metal ion binding protein binding methyltransferase activity transferase activity histone modifying enzyme

参与通路

nucleosome assembly negative regulation of protein kinase activity myotube cell development positive regulation of peptidyl-serine phosphorylation histone lysine methylation establishment of protein localization positive regulation of transcription from RNA polymerase II promoter cellular response to dexamethasone stimulus chromatin modification methylation

SMYD3 Polyclonal Antibody

SMYD3 Antibody (PA5-31919) in ICC/IF

产品信息

PA5-31919

免疫印迹 (WB)

免疫组化 (IHC)

免疫细胞化学 (ICC/IF)

免疫沉淀 (IP)

染色质免疫沉淀实验 (ChIP)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

SMYD3 Polyclonal Antibody

SMYD3 Antibody (PA5-31919) in ICC/IF

产品信息

PA5-31919

免疫印迹 (WB)

免疫组化 (IHC)

免疫细胞化学 (ICC/IF)

免疫沉淀 (IP)

染色质免疫沉淀 实验 (ChIP)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

染色质免疫沉淀实验 (ChIP)