Invitrogen

SMYD4 Polyclonal Antibody

查看其他4个SMYD4抗体

抗体检测数据 (5)

SMYD4 Antibody in Immunocytochemistry (ICC/IF)

SMYD4 Antibody in Immunocytochemistry (ICC/IF)

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SMYD4 Antibody (PA5-96631) in ICC/IF

Immunofluorescence analysis of SMYD4 in A-549 cells. Samples were incubated with SMYD4 Polyclonal antibody (Product # PA5-96631). Blue: DAPI for nuclear staining.

SMYD4 Antibody in Immunocytochemistry (ICC/IF)

SMYD4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SMYD4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SMYD4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SMYD4 Antibody in Western Blot (WB)

Product Image Gallery

SMYD4 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of SMYD4 in A-549 cells. Samples were incubated with SMYD4 Polyclonal antibody (Product # PA5-96631). Blue: DAPI for nuclear staining.

SMYD4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of SMYD4 in paraffin-embedded human colon carcinoma. Samples were incubated with SMYD4 Polyclonal antibody (Product # PA5-96631) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

SMYD4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of SMYD4 in paraffin-embedded mouse lung. Samples were incubated with SMYD4 Polyclonal antibody (Product # PA5-96631) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

SMYD4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of SMYD4 in paraffin-embedded rat spleen. Samples were incubated with SMYD4 Polyclonal antibody (Product # PA5-96631) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

SMYD4 Antibody in Western Blot (WB) — Western blot analysis of SMYD4 in HeLa cells. Samples were incubated with SMYD4 Polyclonal antibody (Product # PA5-96631) using a dilution of 1:2,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

产品信息

PA5-96631

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:5,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:50-1:100

-

酶联免疫吸附实验 (ELISA)

1 µg/mL

-

免疫沉淀 (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

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产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human SMYD4 (NP_4431602)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.27 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2808433

产品详细信息

Immunogen sequence: MDLPVDEWKS YLLQKWASLP TSVQVTISTA ETLRDIFLHS SSLLQPEDEL FLKRLSKGYL VGKDSDAPLF YREEGNKKFQ EKDYTGAAVL YSKGVSHSRP NTEDMSLCHA NRSAALFHLG QYETCLKDIN RAQTHGYPER LQPKIMLRKA ECLVALGRLQ EASQTISDLE RNFTATPALA DVLPQTLQRN LHRLKMKMQE KDSLTESFPA ALAKTLEDAA LREENEQLSN ASSSIGLCVD PLKGRCLVAT KDILPGELLV; Positive Samples: Mouse kidney, Mouse brain, Mouse ovary, Rat kidney, Rat brain

靶标信息

The specific function of the protein remains unknown.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: SET and MYND domain-containing protein 4

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基因别名: G430029E23Rik; KIAA1936; SMYD4; ZMYND21

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UniProt ID: (Human) Q8IYR2, (Mouse) Q8BTK5

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Entrez Gene ID: (Human) 114826, (Mouse) 319822, (Rat) 287525

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methyltransferase activity metal ion binding rRNA (adenine-N6,N6-)-dimethyltransferase activity molecular_function protein C-terminal carboxyl O-methyltransferase activity tRNA (guanine-N2-)-methyltransferase activity C-methyltransferase activity N-methyltransferase activity O-methyltransferase activity S-methyltransferase activity RNA methyltransferase activity mRNA methyltransferase activity tRNA methyltransferase activity protein methyltransferase activity 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity rRNA methyltransferase activity rRNA (uridine-2'-O-)-methyltransferase activity rRNA (adenine-N6-)-methyltransferase activity DNA-methyltransferase activity tRNA (guanine-N1-)-methyltransferase activity rRNA (cytosine-C5-)-methyltransferase activity selenocysteine methyltransferase activity arginine N-methyltransferase activity protein-arginine N-methyltransferase activity lysine N-methyltransferase activity protein-lysine N-methyltransferase activity tRNA (uracil) methyltransferase activity tRNA (guanine) methyltransferase activity tRNA (adenine) methyltransferase activity tRNA (cytosine) methyltransferase activity tRNA (cytosine-5-)-methyltransferase activity tRNA (adenine-N1-)-methyltransferase activity rRNA (adenine) methyltransferase activity rRNA (cytosine) methyltransferase activity rRNA (guanine) methyltransferase activity rRNA (uridine) methyltransferase activity transferase activity protein C-terminal leucine carboxyl O-methyltransferase activity 1-phenanthrol methyltransferase activity protein-arginine N5-methyltransferase activity methylarsonite methyltransferase activity dimethylarsinite methyltransferase activity 4,5-dihydroxybenzo(a)pyrene methyltransferase activity 1-hydroxypyrene methyltransferase activity 1-hydroxy-6-methoxypyrene methyltransferase activity demethylmenaquinone methyltransferase activity cobalt-precorrin-6B C5-methyltransferase activity cobalt-precorrin-7 C15-methyltransferase activity cobalt-precorrin-5B C1-methyltransferase activity cobalt-precorrin-3 C17-methyltransferase activity dimethylamine methyltransferase activity hydroxyneurosporene-O-methyltransferase activity tRNA (adenine-57, 58-N(1)-) methyltransferase activity methylamine-specific methylcobalamin:coenzyme M methyltransferase activity trimethylamine methyltransferase activity methanol-specific methylcobalamin:coenzyme M methyltransferase activity monomethylamine methyltransferase activity P-methyltransferase activity Se-methyltransferase activity 2-phytyl-1,4-naphthoquinone methyltransferase activity tRNA (uracil-2'-O-)-methyltransferase activity tRNA (cytosine-2'-O-)-methyltransferase activity phosphomethylethanolamine N-methyltransferase activity tRNA (cytosine-3-)-methyltransferase activity rRNA (cytosine-2'-O-)-methyltransferase activity rRNA (cytosine-N4-)-methyltransferase activity trihydroxyferuloyl spermidine O-methyltransferase activity

biological_process methylation