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SNAI1 Antibody (600-401-MM5) in ICC/IF
Immunofluorescence of Rabbit Anti-SNAI1 Antibody. Cell Line: NIH-3T3 cells. Fixative: 4% PFA. Permeabilization: 0.3% Triton X-100. Primary Antibody: Anti-SNAI1 at 15µg/mL overnight at 2-8°C. Secondary Antibody: Donkey Anti-Rabbit IgG DyLight™488 (p/n 611-741-127) at 5µL/mL for 1hr at RT. Nuclear Counterstain: DAPI. Staining: (A). DAPI. (B). SNAI1 + DyLight™488 secondary. (C). Merge A+B. (D). secondary only. Localization expected: nucleus, cytosol.
产品信息
600-401-MM5
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Anti-SNAI1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human SNAI1 conjugated to Keyhole Limpet Hemocyanin (KLH).
偶联物
形式
Liquid
浓度
1.03 mg/mL
纯化类型
Affinity chromatography
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
内含物
0.01% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against human SNAI1. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to human and mouse.
靶标信息
Zinc finger protein SNAI1 (SNAIL)is a zinc finger transcriptional repressor which down regulates the expression of the ectodermal gene within the mesoderm and functions to form the mesoderm and neural crest. It is reported that SNAI1 acts as an important factor of tumor invasion as evidenced by its role in E-cadherin down-regulation and induction of epithelial-mesenchymal transition (EMT). In human breast cancer, the expression of SNAI1 and/or the homologous SNAI2 (Slug) has been associated with E-cadherin repression, local or distant metastasis, tumor recurrence, or poor prognosis in different tumor series. It is also reported that Snail 1 protein in the stromamay be anew putative prognosis marker for colon tumors.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Protein sna; Protein snail homolog 1; Sna protein; snai; snail 1; snail 1 homolog; snail 1 zinc finger protein; snail 1, zinc finger protein; snail family zinc finger 1; snail homolog 1; Zinc finger protein SNAI1
基因别名: AI194338; dJ710H13.1; SLUGH2; SNA; Sna1; SNAH; SNAI1; SNAIL; SNAIL1
UniProt ID: (Human) O95863, (Mouse) Q02085
Entrez Gene ID: (Human) 6615, (Mouse) 20613
RNA polymerase II regulatory region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
protein binding
kinase binding
metal ion binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
nucleic acid binding
DNA binding
transcription factor activity, sequence-specific DNA binding
sequence-specific DNA binding
C2H2 zinc finger transcription factor
negative regulation of transcription from RNA polymerase II promoter
osteoblast differentiation
mesoderm formation
epithelial to mesenchymal transition
epithelial to mesenchymal transition involved in endocardial cushion formation
positive regulation of epithelial to mesenchymal transition
negative regulation of vitamin D biosynthetic process
cell migration
positive regulation of cell migration
hair follicle morphogenesis
negative regulation of DNA damage response, signal transduction by p53 class mediator
positive regulation of transcription, DNA-templated
palate development
cartilage morphogenesis
trophoblast giant cell differentiation
negative regulation of cell differentiation involved in embryonic placenta development
left/right pattern formation
Notch signaling involved in heart development
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage
regulation of bicellular tight junction assembly
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
multicellular organism development
mesoderm development
negative regulation of transcription, DNA-templated
