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SNAP alpha/beta Antibody (PA5-80840) in ICC/IF
Immunofluorescence staining of SNAP alpha/beta in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with SNAP alpha/beta Polyclonal Antibody (Product # PA5-80840, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). P... View More
产品信息
PA5-80840
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant Human SNAP-alpha Protein
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A, Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2788109
产品详细信息
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Human SNAP-alpha.
靶标信息
SNAPs (soluble NSF attachment proteins), acting in concert with SNAREs ( SNAP receptors) and the N-ethylmaleimide-sensitive fusion protein (NSF) are required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-toendosome fusion and transcytotic vesicles-to-plasma membrane transport. Vesicle-to-target membrane docking (initial contact) occurs when the vesicle SNARE binds to its cognate target membrane SNARE. a-SNAP (or b-SNAP in brain) then binds to this docking complex and mediates the binding of NSF and thus the formation of a 20S fusion particle. It is thought that, once NSF is bound, ATP hydrolysis by NSF initiates the fusion process.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: alpha snap; alpha soluble NSF attachment protein; alpha-SNAP; Alpha-soluble NSF attachment protein; N-ethylmaleimide-sensitive factor attachment protein alpha; N-ethylmaleimide-sensitive factor attachment protein, alpha; SNAP-alpha
基因别名: NAPA; SNAPA
UniProt ID: (Human) P54920
Entrez Gene ID: (Human) 8775
intracellular protein transport
ER to Golgi vesicle-mediated transport
retrograde vesicle-mediated transport, Golgi to ER
intra-Golgi vesicle-mediated transport
brain development
regulation of synaptic vesicle priming
neuron differentiation
synaptic transmission, glutamatergic
SNARE complex disassembly
apical protein localization
COPII vesicle coating
membrane fusion
