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SNAP23 Antibody (PA1-738) in ICC/IF
Immunofluorescence analysis of SNAP-23 was performed using 90% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SNAP23 Rabbit Polyclonal Antibody (Product # PA1-738) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product... View More
产品信息
PA1-738
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Bovine,
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Sythetic Peptide: C N(193) K N R I D I A N T R A K K L I D S(210)
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2192025
产品详细信息
PA1-738 detects synaptosomal-associated protein 23 (SNAP-23) from human, mouse and rat samples.
PA1-738 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects an ~23 kDa protein representing SNAP-23 from rat/mouse brain protein extract and HeLa cell lysates.
PA1-738 immunizing peptide corresponds to amino acid residues 193-210 from mouse SNAP-23 protein. PA1-738 immunizing peptide (Cat. # PEP-147) is available for use in neutralization and control experiments.
靶标信息
Specificity of vesicular transport is regulated, in part, by the interaction of a vesicle-associated membrane protein termed synaptobrevin/VAMP with a target compartment membrane protein termed syntaxin. These proteins, together with SNAP25 (synaptosome-associated protein of 25 kDa), form a complex which serves as a binding site for the general membrane fusion machinery. Synaptobrevin/VAMP and syntaxin are believed to be involved in vesicular transport in most, if not all cells, while SNAP25 is present almost exclusively in the brain, suggesting that a ubiquitously expressed homolog of SNAP25 exists to facilitate transport vesicle/target membrane fusion in other tissues. The protein encoded by this gene is structurally and functionally similar to SNAP25 and binds tightly to multiple syntaxins and synaptobrevins/VAMPs. It is an essential component of the high affinity receptor for the general membrane fusion machinery and is an important regulator of transport vesicle docking and fusion. Two alternative transcript variants encoding different protein isoforms have been described for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: OTTHUMP00000161263; SNAP-23; Synaptosomal-associated protein 23; synaptosomal-associated protein 23 kD; synaptosomal-associated protein, 23 kD; synaptosomal-associated protein, 23kD; synaptosomal-associated protein, 23kDa; synaptosome associated protein 23kDa; Syndet; Vesicle-membrane fusion protein SNAP-23
基因别名: 23kDa; AA408749; HsT17016; SNAP-23; SNAP23; SNAP23A; SNAP23B; Sndt; Syndet
UniProt ID: (Human) O00161, (Mouse) O09044, (Rat) O70377
Entrez Gene ID: (Human) 8773, (Mouse) 20619, (Rat) 64630
histamine secretion by mast cell
protein complex assembly
transport
exocytosis
protein transport
synaptic vesicle priming
vesicle-mediated transport
regulation of exocytosis
synaptic vesicle fusion to presynaptic active zone membrane
membrane fusion
post-Golgi vesicle-mediated transport
vesicle targeting
