Invitrogen

SNF1 Polyclonal Antibody

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SNF1 Antibody (PA5-144419) in WB

Western blot analysis of SNF1 in recombinant protein using a dilution of 1:2,000. Samples were incubated with SNF1 polyclonal antibody (Product # PA5-144419). Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 27 kDa. Observed band size: 36 kDa.

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产品信息

PA5-144419

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

酶联免疫吸附实验 (ELISA)

Assay-dependent

产品规格

种属反应

Yeast

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant Saccharomyces cerevisiae (strain ATCC 204508/S288c) (Baker's yeast) SNF1 protein (435-633aa)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

3.91 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.03% Proclin 300

保存条件

-20°C or -80°C if preferred

运输条件

Wet ice

AB_3091714

靶标信息

Serine/threonine protein kinase essential for release from glucose repression (PubMed:3526554, PubMed:6366512, PubMed:3049551, PubMed:1944227, PubMed:8289797, PubMed:8628258, PubMed:25869125). Catalytic subunit of the AMP-activated protein kinase complex also known as the SNF1 kinase complex (Snf1c), a central regulator of cellular energy homeostasis, which, in response to a fall in intracellular ATP levels, activates energy-producing pathways and inhibits energy-consuming processes (PubMed:8289797, PubMed:26667037). The complex phosphorylates histone H3 to form H3S10ph, which promotes H3K14ac formation, leading to transcriptional activation through TBP recruitment to the promoters (PubMed:15719021). The complex also negatively regulates the HOG1 MAPK pathway in ER stress response including unfolded protein response (UPR) (PubMed:25730376, PubMed:26394309). Under nutrient/energy depletion, the complex phosphorylates and activates PAS kinase PSK1 which in turn activates PBS1, leading to the inhibition of the TORC1 signaling pathway (PubMed:25428989). SNF1 also interacts and phosphorylates adenylate cyclase CYR1 and negatively regulates the protein kinase A signaling pathway (PubMed:26309257). Also phosphorylates and regulates the transcriptional activator CAT8 (PubMed:15121831).

仅用于科研。不用于诊断过程。未经明确授权不得转售。