Bethyl Laboratories

SOS2 Polyclonal Antibody

查看其他7个SOS2抗体

抗体检测数据 (3)

SOS2 Antibody in Western Blot (WB)

SOS2 Antibody in Western Blot (WB)

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SOS2 Antibody (A301-893A) in WB

Detection of human SOS2 by western blot. Samples: Whole cell lysate (25 µg) from Jurkat, RKO, CAL-27, PANC-1, and MDA-MB-231 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SOS2 antibody (Product # A301-893A lot 3) used for WB at 0.4 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

SOS2 Antibody in Western Blot (WB)

SOS2 Antibody in Western Blot (WB)

SOS2 Antibody in Immunoprecipitation (IP)

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SOS2 Antibody in Western Blot (WB) — Detection of human SOS2 by western blot. Samples: Whole cell lysate (25 µg) from Jurkat, RKO, CAL-27, PANC-1, and MDA-MB-231 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SOS2 antibody (Product # A301-893A lot 3) used for WB at 0.4 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

SOS2 Antibody in Western Blot (WB) — Detection of mouse SOS2 by western blot. Samples: Whole cell lysate (25 µg) from CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SOS2 antibody (Product # A301-893A lot 3) used for WB at 0.4 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

SOS2 Antibody in Immunoprecipitation (IP) — Detection of human SOS2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SOS2 antibody (Product # A301-893A) (lot 3) used for IP at 6 µg per reaction. SOS2 was also immunoprecipitated by a previous lot of this antibody (Product # A301-893A) (lot 2) and a second antibody against a different epitope of SOS2 (BL7525). For blotting immunoprecipitated SOS2, A301-893A was used at 0.4 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds.

产品信息

A301-893A

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000-1:10,000

-

免疫沉淀 (IP)

2-10 µg/mg lysate

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Region between residue 1282 and 1332 of Human son of sevenless ortholog 2.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

tris citrate/phosphate, pH 7-8

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Wet ice

产品详细信息

The recommended shelf life for this product is 1 year from date of receipt.

靶标信息

SOS2 promotes the exchange of Ras-bound GDP by GTP.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Son of sevenless homolog 2; SOS-2

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基因别名: mSOS-2; NS9; SOS-2; SOS2

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UniProt ID: (Human) Q07890, (Mouse) Q02384

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Entrez Gene ID: (Human) 6655, (Mouse) 20663

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DNA binding guanyl-nucleotide exchange factor activity Ras guanyl-nucleotide exchange factor activity Rho guanyl-nucleotide exchange factor activity protein binding protein heterodimerization activity guanyl-nucleotide exchange factor

B cell homeostasis lymphocyte homeostasis small GTPase mediated signal transduction regulation of T cell differentiation in thymus regulation of Rho protein signal transduction regulation of T cell proliferation positive regulation of GTPase activity positive regulation of small GTPase mediated signal transduction regulation of pro-B cell differentiation