Invitrogen

SOX17 Recombinant Rabbit Monoclonal Antibody (6H42L1)

SOX17 Antibody in Immunocytochemistry (ICC/IF)

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SOX17 Antibody (703063) in ICC/IF

For immunofluorescence analysis, iPS cells and iPS cells differentiated to definitive endoderm were fixed and permeabilized for detection of endogenous SOX17 using Anti-SOX17 Recombinant Rabbit Monoclonal Antibody (Product # 703063, 1:100) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962) and cytoskeletal F-actin (red) staining using Rhodamine Phalloidin (... View More

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SOX17 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Expression of SOX17 was observed specifically in IPS cells differentiated to definitive endoderm in comparison to IPS cells using Anti-SOX17 Recombinant Rabbit Monoclonal Antibody (Product # 703063) in Immunofluorescence. {RE}

产品信息

703063

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

6H42L1

抗原

Protein corresponding to human Sox17 [aa177-aa414]

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2784556

产品详细信息

This antibody is predicted to react with Monkey, Cat, Bovine.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

SOX17 (transcription factor SOX-17) belongs to the SRY-related HMB box family of transcription factors. It acts as a transcription regulator that binds target promoter DNA and bends the DNA. It binds to the sequences 5'-AACAAT-'3 or 5'-AACAAAG-3'. SOX17 modulates transcriptional regulation via WNT3A and inhibits Wnt signaling. It promotes degradation of activated CTNNB1. SOX17 plays an essential role in the regulation of embryonic development and is required for normal looping of the embryonic hear tube and for normal development of the definitive gut endoderm. Mutations in the gene can result in vesicoureteral reflux.

仅用于科研。不用于诊断过程。未经明确授权不得转售。