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SP100 Antibody (PA5-96447) in WB
Western blot analysis of SP100 in extracts of various cell lines. Samples were incubated with SP100 Polyclonal antibody (Product # PA5-96447) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
产品信息
PA5-96447
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 181-480 of human SP100 (NP_0011936311)
偶联物
形式
Liquid
浓度
0.15 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2808249
产品详细信息
Immunogen sequence: FRSLTWPPSG SPSHAGTTPP ENGLSEHPCE TEQINAKRKD TTSDKDDSLG SQQTNEQCAQ KAEPTESCEQ IAVQVNNGDA GREMPCPLPC DEESPEAELH NHGIQINSCS VRLVDIKKEK PFSNSKVECQ AQARTHHNQA SDIIVISSED SEGSTDVDEP LEVFISAPRS EPVINNDNPL ESNDEKEGQE ATCSRPQIVP EPMDFRKLST FRESFKKRVI GQDHDFSESS EEEAPAEASS GALRSKHGEK APMTSRSTST WRIPSRKRRF SSSDFSDLSN GEELQETCSS SLRRGSGKED; Positive Samples: MCF7, HeLa, 293T, HepG2, Mouse spleen, Mouse thymus, Mouse lung, Mouse heart, Mouse liver; Cellular Location: Cytoplasm, Nucleus, PML body
靶标信息
This gene encodes a subnuclear organelle and major component of the PML (promyelocytic leukemia)-SP100 nuclear bodies. PML and SP100 are covalently modified by the SUMO-1 modifier, which is considered crucial to nuclear body interactions. The encoded protein binds heterochromatin proteins and is thought to play a role in tumorigenesis, immunity, and gene regulation. Alternatively spliced variants have been identified for this gene; one of which encodes a high-mobility group protein.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DKFZp686E07254; FLJ00340; FLJ34579; Nuclear autoantigen Sp-100; Nuclear dot-associated Sp100 protein; SP 100; SP100-HMG nuclear autoantigen; Speckled 100 kDa
基因别名: A430075G10Rik; lysp100b; SP100
UniProt ID: (Human) P23497, (Mouse) O35892
Entrez Gene ID: (Human) 6672, (Mouse) 20684
negative regulation of transcription from RNA polymerase II promoter
telomere maintenance
transcription, DNA-templated
inflammatory response
DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator
negative regulation of endothelial cell migration
viral process
protein sumoylation
response to type I interferon
response to interferon-gamma
negative regulation of DNA binding
negative regulation of sequence-specific DNA binding transcription factor activity
regulation of angiogenesis
positive regulation of transcription, DNA-templated
negative regulation of protein export from nucleus
negative regulation of cellular component movement
interferon-gamma-mediated signaling pathway
type I interferon signaling pathway
regulation of extrinsic apoptotic signaling pathway via death domain receptors
regulation of Fas signaling pathway
chromatin remodeling
regulation of transcription, DNA-templated
