Invitrogen

SPARC Monoclonal Antibody (5031)

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SPARC Antibody (MA1-43027) in WB

Western blot was performed using Anti-SPARC Monoclonal Antibody (5031) (Product # MA1-43027) and a 40 kDa band corresponding to SPARC was observed in BJ, U-2 OS, U-87 MG and SH-SY5Y which are reported to be positive and not in other cell lines like MCF7, HT-29 and Raji. Whole cell extracts (30 µg lysate) of BJ (Lane 1), U-2 OS (Lane 2), U-87 MG (Lane 3), SH-SY5Y (Lane 4), MCF7 (Lane 5), HT-29 (Lane 6) and Raji (Lane 7) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred... View More

SPARC Antibody in Immunohistochemistry (IHC)

Product Image Gallery

SPARC Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of SPARC was observed in BJ, U-2 OS, U-87 MG and SH-SY5Y which are reported to be positive and not in other cell lines like MCF7, HT-29 and Raji using Anti-SPARC Monoclonal Antibody (5031) (Product # MA1-43027) in Western Blot. {RE}

产品信息

MA1-43027

应用

建议稀释比

已发表文章

免疫印迹 (WB)

5-10 µg/mL

免疫组化 (IHC)

5-10 µg/mL

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酶联免疫吸附实验 (ELISA)

5-10 µg/mL

放射免疫测定 (RIA)

5-10 µg/mL

产品规格

种属反应

Bovine, Human, Rat

已发表种属

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

5031

抗原

Human osteonectin.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

9.7 mg/mL

纯化类型

SDS-PAGE

保存液

50% water with 50% glycerol

内含物

no preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2195095

产品详细信息

MA1-43027 detects SPARC in human, rat and bovine samples.

MA1-43027 has successfully been used in radioimmunoassays, Western blot, ELISA and immunohistochemistry applications.

MA1-43027 was derived from human platelet osteonectin.

靶标信息

SPARC gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Generation of Osteosarcomas from a Combination of Rb Silencing and c-Myc Overexpression in Human Mesenchymal Stem Cells.

Stem cells translational medicine

Wang JY,Wu PK,Chen PC,Lee CW,Chen WM,Hung SC

MA1-43027 was used in Immunohistochemistry to transform human mesenchymal stem cells (MSCs) into Osteosarcoma cells through a combination of retinoblastoma (Rb) knockdown and c-Myc overexpression.

Wed Feb 01 00:00:00 UTC 2017

Generation of Osteosarcomas from a Combination of Rb Silencing and c-Myc Overexpression in Human Mesenchymal Stem Cells.

Stem cells translational medicine

Wang JY,Wu PK,Chen PC,Lee CW,Chen WM,Hung SC

MA1-43027 was used in Immunohistochemistry to transform human mesenchymal stem cells (MSCs) into Osteosarcoma cells through a combination of retinoblastoma (Rb) knockdown and c-Myc overexpression.

Wed Feb 01 00:00:00 UTC 2017

生物信息学

蛋白别名: Basement membrane protein 40; Basement-membrane protein 40; BM-40; BM40; Cysteine rich protein; ON; Osteonectin; Secreted acidic cystein rich glycoprotein; Secreted acidic cystein-rich glycoprotein (osteonectin); secreted acidic cysteine rich glycoprotein; Secreted protein acidic and rich in cysteine; Secreted protein acidic and rich in cysteine (SPARC); secreted protein, acidic, cysteine-rich (osteonectin); SPARC

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基因别名: BM-40; BOS_8355; OI17; ON; SPARC

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UniProt ID: (Bovine) P13213, (Human) P09486, (Rat) P16975

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Entrez Gene ID: (Bovine) 282077, (Human) 6678, (Rat) 24791

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功能

calcium ion binding extracellular matrix binding protein binding collagen binding

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