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SPHK1 Antibody (PA5-87359) in WB
Western blot analysis of SPHK1 in extracts of various cell lines. Samples were incubated with SPHK1 Polyclonal antibody (Product # PA5-87359) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Product Details
PA5-87359
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 50-384 of human SPHK1 (NP_0011360741)
Conjugate
Form
Liquid
Concentration
1.35 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2804091
Product Specific Information
Immunogen sequence: TLMLTERRNH ARELVRSEEL GRWDALVVMS GDGLMHEVVN GLMERPDWET AIQKPLCSLP AGSGNALAAS LNHYAGYEQV TNEDLLTNCT LLLCRRLLSP MNLLSLHTAS GLRLFSVLSL AWGFIADVDL ESEKYRRLGE MRFTLGTFLR LAALRTYRGR LAYLPVGRVG SKTPASPVVV QQGPVDAHLV PLEEPVPSHW TVVPDEDFVL VLALLHSHLG SEMFAAPMGR CAAGVMHLFY VRAGVSRAML LRLFLAMEKG RHMEYECPYL VYVPVVAFRL EPKDGKGVFA VDGELMVSEA VQGQVHPNYF WMVSGCVEPP PSWKPQQMPP PEEPL; Positive Samples: SW480, HeLa, Mouse kidney; Cellular Location: Cell membrane, Cytoplasm, Nucleus
Target Information
SPHK1, along with SPHK2, catalyzes the phosphorylation of sphingosine producing the signaling molecule sphingosine-1-phosphate (S1P). S1P signals through G-protein coupled receptors and controls diverse cellular processes. Inhibitors of SPHKs are viewed as potential therapeutics for the treatment of cancer. SPHK1 catalyzes the phosphorylation of sphingosine to sphingosine 1 phosphate (S1P), an important signaling molecule with intra- and extracellular functions. Inside the cell S1P acts as a signaling molecule like other sphingolipid metabolites like ceramide and sphingosine. S1P has been implicated in regulating cell differentiation, calcium mobilization from intracellular stores, and apoptosis. The cell surface receptors for S1P are the EDG family of G protein-coupled receptors (S1P Receptors).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Acetyltransferase SPHK1; RP23-193A16.4; SK 1; SKase; Sphingosine kinase 1; SPK 1
Gene Aliases: 1110006G24Rik; SK1; SPHK; SPHK1; SPK; Spk1
UniProt ID: (Human) Q9NYA1, (Mouse) Q8CI15
Entrez Gene ID: (Human) 8877, (Mouse) 20698
blood vessel development
positive regulation of neurotransmitter secretion
sphingosine-1-phosphate signaling pathway
protein folding
sphingosine metabolic process
inflammatory response
signal transduction
brain development
female pregnancy
cellular response to starvation
positive regulation of peptidyl-threonine phosphorylation
regulation of tumor necrosis factor-mediated signaling pathway
positive regulation of neuron projection development
response to amine
cyclooxygenase pathway
calcium-mediated signaling
sphingolipid biosynthetic process
positive regulation of cell growth
positive regulation of cell migration
positive regulation of protein ubiquitination
response to magnesium ion
response to progesterone
regulation of interleukin-1 beta production
response to ATP
intracellular signal transduction
positive regulation of NF-kappaB import into nucleus
negative regulation of apoptotic process
positive regulation of angiogenesis
positive regulation of mitotic cell cycle
positive regulation of smooth muscle contraction
sphingosine biosynthetic process
sphingoid catabolic process
lipid phosphorylation
positive regulation of fibroblast proliferation
positive regulation of NF-kappaB transcription factor activity
cellular response to hydrogen peroxide
response to interleukin-1
cellular response to growth factor stimulus
positive regulation of protein phosphorylation
positive regulation of cell proliferation
phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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