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SPR Antibody (PA5-80068) in WB
Western blot analysis of SPR in Lane 1: human HepG2 whole cell lysate, Lane 2: mouse HEPA1-6 whole cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with SPR polyclonal antibody (Product # PA5-80068) at a dilution of 0.5 µg/mL (overnight at 4°C), followed... View More
Product Details
PA5-80068
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human SPR recombinant protein (Position: V36-K261).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2747183
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human HepG2 whole cell, mouse HEPA1-6 whole cell.
Target Information
SPR encodes an aldo-keto reductase that catalyzes the NADPH-dependent reduction of pteridine derivatives and is important in the biosynthesis of tetrahydrobiopterin (BH4). Mutations in this gene result in DOPA-responsive dystonia due to sepiaterin reductase deficiency. A pseudogene has been identified on chromosome 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Sepiapterin reductase; short chain dehydrogenase/reductase family 38C, member 1; SPR
Gene Aliases: AA409688; ENSMUSG00000071345; Gm10328; SDR38C1; SPR
UniProt ID: (Human) P35270
Entrez Gene ID: (Human) 6697, (Mouse) 20751
L-phenylalanine metabolic process
tetrahydrobiopterin biosynthetic process
nitric oxide biosynthetic process
metabolic process
response to organic substance
obsolete death
pteridine metabolic process
regulation of multicellular organism growth
norepinephrine metabolic process
dopamine metabolic process
serotonin metabolic process
tetrahydrobiopterin metabolic process
cell morphogenesis involved in neuron differentiation
voluntary musculoskeletal movement
oxidation-reduction process
regulation of nitric-oxide synthase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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