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SPT6 Antibody (PA5-115959) in ICC/IF
Immunocytochemistry analysis of SPT6 in HepG2 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115959). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.
产品信息
PA5-115959
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human SUPT6H(Accession Q7KZ85), corresponding to amino acid residues A1678-R1726.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2900593
产品详细信息
Antibody detects endogenous levels of total Spt6.
靶标信息
Transcription elongation factor which binds histone H3 and plays a key role in the regulation of transcription elongation and mRNA processing. Enhances the transcription elongation by RNA polymerase II (RNAPII) and is also required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. Besides chaperoning histones in transcription, acts to transport and splice mRNA by forming a complex with IWS1 and the C-terminal domain (CTD) of the RNAPII subunit RPB1 (POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes (such as SETD2), to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription. SUPT6H via its association with SETD1A, regulates both class-switch recombination and somatic hypermutation through formation of H3K4me3 epigenetic marks on activation-induced cytidine deaminase (AICDA) target loci. Promotes the activation of the myogenic gene program by entailing erasure of the repressive H3K27me3 epigenetic mark through stabilization of the chromatin interaction of the H3K27 demethylase KDM6A.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Histone chaperone suppressor of Ty6; hSPT6; MGC87943; suppressor of Ty 6 homolog; Tat-cotransactivator 2 protein; Tat-CT2 protein; Transcription elongation factor SPT6
基因别名: 5131400N11Rik; AI132449; emb-5; KIAA0162; mKIAA0162; SPT6; SPT6H; Supt6; SUPT6H
UniProt ID: (Human) Q7KZ85, (Mouse) Q62383
Entrez Gene ID: (Human) 6830, (Mouse) 20926, (Rat) 303281
chromatin remodeling
transcription, DNA-templated
regulation of transcription, DNA-templated
mRNA processing
RNA splicing
regulation of mRNA export from nucleus
viral process
positive regulation of transcription elongation from RNA polymerase II promoter
regulation of isotype switching
regulation of mRNA processing
mRNA transport
regulation of muscle cell differentiation
negative regulation of histone H3-K27 methylation
nucleobase-containing compound metabolic process
regulation of transcription from RNA polymerase II promoter
transport
regulation of DNA-templated transcription, elongation
