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SPTLC1 Antibody (PA5-121032) in WB
Western blot analysis of SPTLC1 in extracts of various cell lines. Samples were incubated with SPTLC1 Polyclonal antibody (Product # PA5-121032) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-121032
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 37-310 of human SPTLC1 (NP_006406.1).
Conjugate
Form
Liquid
Concentration
1.29 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914604
Product Specific Information
Positive test controls include: SKOV3, MCF7, HepG2, Mouse kidney, Mouse lung, Mouse liver, Mouse thymus, Rat lung, Rat kidney. The target is usually found in the following locations: Endoplasmic reticulum membrane, Single-pass membrane protein.
Immunogen sequence: RLLFSKTYKL QERSDLTVKE KEELIEEWQP EPLVPPVPKD HPALNYNIVS GPPSHKTVVN GKECINFASF NFLGLLDNPR VKAAALASLK KYGVGTCGPR GFYGTFDVHL DLEDRLAKFM KTEEAIIYSY GFATIASAIP AYSKRGDIVF VDRAACFAIQ KGLQASRSDI KLFKHNDMAD LERLLKEQEI EDQKNPRKAR VTRRFIVVEG LYMNTGTICP LPELVKLKYK YKARIFLEES LSFGVLGEHG RGVTEHYGIN IDDIDLISAN MENA
Target Information
Serine palmitoyltransferase (SPT) is the key enzyme in sphingolipid biosynthesis. It catalyzes the pyridoxal-5-prime-phosphate-dependent condensation of L-serine and palmitoyl-CoA to 3-oxosphinganine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hLCB1; HSAN; LCB 1; Long chain base biosynthesis protein 1; MGC14645; RP11-118F2.1; serine C-palmitoyltransferase; Serine palmitoyltransferase 1; serine palmitoyltransferase subunit 1; serine palmitoyltransferase, long chain base subunit 1; Serine-palmitoyl-CoA transferase 1; SPT 1; SPT1
Gene Aliases: AW552086; C77762; E030036H05; HSAN1; HSN1; LBC1; LCB1; rCG_44191; RGD1306617; SPT1; SPTI; SPTLC1
UniProt ID: (Human) O15269, (Mouse) O35704, (Rat) D4A2H2
Entrez Gene ID: (Human) 10558, (Mouse) 268656, (Rat) 361213
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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