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SRC2 Antibody (PA1-846) in ICC/IF
Immunofluorescence analysis of GRIP1 was done on 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with GRIP1 Rabbit Polyclonal Antibody (Product # PA1-846) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at... View More
产品信息
PA1-846
应用
建议稀释比
已发表文章
产品规格
种属反应
Hamster,
Human
已发表种属
Human,
Non-human primate,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to residues P(206) L P D S E E E G H D S(217) C of human GRIP1.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2112760
产品详细信息
PA1-846 detects glucocorticoid receptor interacting protein-1 (GRIP1) from hamster and human cells.
PA1-846 has been successfully used in immunocytochemistry, and immunofluorescence procedures. Immunocytochemical staining of GRIP1 in CHO cells with PA1-846 results in cytoplasmic and nuclear staining. This antibody has not been used successfully in Western blot procedures.
PA1-846 immunogen is a synthetic peptide corresponding to residues P(206) L P D S E E E G H D S(217) C of human GRIP1. The PA1-846 immunizing peptide (Cat. # PEP-050) is available for use in neutralization and control experiments.
靶标信息
SRC2 (steroid receptor coactivator 2), also known as nuclear receptor coactivator2, NCOA2 or TIF2, aids in the function of nuclear hormone receptors. Nuclear hormone receptors are conditional transcription factors that play important roles in various aspects of cell growth, development, and homeostasis by controlling expression of specific genes. Members of the nuclear hormone receptor superfamily, which includes the 5 steroid receptors and class II nuclear receptors, are structurally characterized by 3 distinct domains: an N-terminal transcriptional activation domain, a central DNA-binding domain, and a C-terminal hormone-binding domain. Before the binding of hormone, steroid receptors, which are sometimes called class I of the nuclear hormone receptor family, remain inactive in a complex with heat-shock protein-90 and other stress family proteins. Binding of hormone induces critical conformational changes in steroid receptors that cause them to dissociate from the inhibitory complex, bind as homodimers to specific DNA enhancer elements associated with target genes, and modulate that gene's transcription. After binding to enhancer elements, transcription factors require transcriptional coactivator proteins to mediate their stimulation of transcription initiation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: bHLHe75; c-fgr; c-src2; Class E basic helix-loop-helix protein 75; FLJ43153; glucocorticoid receptor-interacting protein-1; hTIF2; MGC75096; NCoA-2; Nuclear receptor coactivator 2; p160 steroid receptor coactivator 2; p55c-fgr; p58c-fgr; TIF 2; Transcriptional intermediary factor 2
基因别名: BHLHE75; GRIP1; I79_019839; KAT13C; NCoA-2; NCOA2; SRC2; TIF2
UniProt ID: (Human) Q15596
Entrez Gene ID: (Human) 10499, (Chinese hamster) 100762955
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding
chromatin binding
transcription coactivator activity
histone acetyltransferase activity
protein binding
ligand-dependent nuclear receptor binding
PDZ domain binding
estrogen receptor binding
ligand-dependent nuclear receptor transcription coactivator activity
thyroid hormone receptor coactivator activity
protein complex binding
progesterone receptor binding
nuclear hormone receptor binding
glucocorticoid receptor binding
retinoid X receptor binding
thyroid hormone receptor binding
protein dimerization activity
DNA polymerase binding
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
male gonad development
regulation of glucose metabolic process
bile acid and bile salt transport
histone acetylation
cerebellum development
intracellular receptor signaling pathway
cellular response to hormone stimulus
circadian regulation of gene expression
cellular lipid metabolic process
locomotor rhythm
positive regulation of female receptivity
positive regulation of transcription from RNA polymerase II promoter
cellular response to Thyroglobulin triiodothyronine
positive regulation of receptor activity
positive regulation of glucocorticoid receptor signaling pathway
