Invitrogen

SRSF1 Polyclonal Antibody

SRSF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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SRSF1 Antibody (PA5-30220) in IHC (P)

SRSF1 Polyclonal Antibody detects SFRS1 protein at nucleus on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. SRSF1 Polyclonal Antibody (Product # PA5-30220) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

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SRSF1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with SRSF1 siRNA and reduction of signal was observed in Western Blot using SRSF1 Polyclonal Antibody (Product # PA5-30220). {KD}

产品信息

PA5-30220

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:10,000

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

免疫沉淀 (IP)

查看1篇已发表文章 1篇已发表文章

RNA免疫共沉淀 (RIP)

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 1 and 224 of Human SF2

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol, 1% BSA

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2547694

产品详细信息

Recommended positive controls: rat brain, HeLa, HepG2, Mouse brain.

Predicted reactivity: Mouse (100%), Rat (100%), Pig (100%), Chicken (98%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

UBE2E2 catalyzes the covalent attachment of ubiquitin to other proteins.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

mRNA recognition and packaging by the human transcription-export complex.

Nature

Pacheco-Fiallos B,Vorländer MK,Riabov-Bassat D,Fin L,O'Reilly FJ,Ayala FI,Schellhaas U,Rappsilber J,Plaschka C

Published figure using SRSF1 polyclonal antibody (Product # PA5-30220) in Western Blot

Sat Apr 01 00:00:00 UTC 2023

circCELF1 Induces the Apoptosis and Autophagy of Astrocytes in Ischemic Stroke via Upregulating NFAT5.

Cerebrovascular diseases (Basel, Switzerland)

Li W,Teng J

PA5-30220 was used in Immunoprecipitation to unveil the role of a novel circRNA hsa_circ_0000304 (termed as circCELF1) and underlying mechanism in ischemic stroke.

Thu Jun 01 00:00:00 UTC 2023

HOTTIP-dependent R-loop formation regulates CTCF boundary activity and TAD integrity in leukemia.

Molecular cell

Luo H,Zhu G,Eshelman MA,Fung TK,Lai Q,Wang F,Zeisig BB,Lesperance J,Ma X,Chen S,Cesari N,Cogle C,Chen B,Xu B,Yang FC,So CWE,Qiu Y,Xu M,Huang S

PA5-30220 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot, ChIP Assay to conclude that HOTTIP-mediated R-loop formation directly reinforces CTCF chromatin boundary activity and TAD integrity to drive oncogene transcription and leukemia development.

Thu Feb 17 00:00:00 UTC 2022

HOTTIP-dependent R-loop formation regulates CTCF boundary activity and TAD integrity in leukemia.

Molecular cell

Luo H,Zhu G,Eshelman MA,Fung TK,Lai Q,Wang F,Zeisig BB,Lesperance J,Ma X,Chen S,Cesari N,Cogle C,Chen B,Xu B,Yang FC,So CWE,Qiu Y,Xu M,Huang S

Thu Feb 17 00:00:00 UTC 2022

An IGF-1R-mTORC1-SRPK2 signaling Axis contributes to FASN regulation in breast cancer.

BMC cancer

McClellan B,Gries P,Harlow B,Tiziani S,Jolly C,deGraffenried L

PA5-30220 was used in RNA Immunoprecipitation to establish a potential IGF-1-mTORC1-SRPK2-FASN axis in breast cancer, which could be a potential therapeutic target for cancers that overexpress FASN and components of the IGF-1R pathway.

Mon Sep 12 00:00:00 UTC 2022

Elucidating the Antiviral Mechanism of Different MARCH Factors.

mBio

Umthong S,Lynch B,Timilsina U,Waxman B,Ivey EB,Stavrou S

PA5-30220 was used in Western Blot to perform a comprehensive analysis on a number of envelope glycoproteins and show that MARCH proteins have broad antiviral functions.

Tue Mar 02 00:00:00 UTC 2021

Moringa oleifera Gold Nanoparticles Modulate Oncogenes, Tumor Suppressor Genes, and Caspase-9 Splice Variants in A549 Cells.

Journal of cellular biochemistry

Tiloke C,Phulukdaree A,Anand K,Gengan RM,Chuturgoon AA

Published figure using SRSF1 polyclonal antibody (Product # PA5-30220) in Western Blot

Sat Oct 01 00:00:00 UTC 2016

生物信息学

蛋白别名: Alternative-splicing factor 1; ASF-1; ASF/SF2; pre-mRNA-splicing factor SF2, P33 subunit; Pre-mRNA-splicing factor SRp30a; Serine/arginine-rich splicing factor 1; splicing factor 2; Splicing factor, arginine/serine-rich 1; splicing factor, arginine/serine-rich 1 (ASF/SF2); splicing factor, arginine/serine-rich 1B; splicing factor, arginine/serine-rich, 30-KD, A; SR splicing factor 1

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基因别名: 1110054N12Rik; 5730507C05Rik; 6330415C05Rik; AI482334; ASF; AW491331; OK/SW-cl.3; SF2; SF2P33; SFRS1; SRp30a; SRSF1

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UniProt ID: (Human) Q07955, (Mouse) Q5SXC5

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Entrez Gene ID: (Human) 6426, (Rat) 689890, (Mouse) 110809

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功能

nucleotide binding RNA binding mRNA binding protein binding poly(A) RNA binding RS domain binding nucleic acid binding RNA splicing factor

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