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SSEA1 Antibody (MA1-35093) in Flow
THP-1 and Ramos cells were fixed and permeabilized using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-000) and then stained with SSEA1 Monoclonal Antibody (BRA4F1) (Product # MA1-35093) (red histogram) at a concentration of 1 µg and CD19 Monoclonal Antibody (HIB19), FITC, eBioscience™ (Product #11-0199-42) (green histogram) at a concentration of 1 µg or left unstained (black histogram), followed by Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor™ Plus 647 (Product # A55060,... View More
产品信息
MA1-35093
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgM
分类
Monoclonal
类型
Antibody
克隆号
BRA4F1
抗原
Erythroid-myeloid leukemia cell line
偶联物
形式
Liquid
浓度
0.1 mg/mL
纯化类型
purified
保存液
PBS
内含物
0.02% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_1071306
产品详细信息
Bra4F1 reacts with CD15 (220 kDa). CD15 is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. CD15 is further expressed in Reed-Sternberg cells in classic Hodgkin’s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes. It is also expressed on a wide variety of other tumor cells including myeloid leukemia, breast, colorectal, and lung cancer cells. Bra4F1 was clustered at the IVth International Workshop on Leucocyte Differentiation Antigens.
靶标信息
CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase; alpha (1,3) fucosyltransferase, myeloid-specific; Alpha-(1,3)-fucosyltransferase 4; ELAM ligand fucosyltransferase; ELAM-1 ligand fucosyltransferase; Fuc-TIV; Fucosyltransferase 4; fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific); Fucosyltransferase IV; fucT-IV; FUTIV; Galactoside 3-L-fucosyltransferase; Lewis X; SSEA-1; stage-specific embryonic antigen 1
基因别名: CD15; ELFT; FCT3A; FUC-TIV; FUT4; FUTIV; LeX; SSEA-1
UniProt ID: (Human) P22083
Entrez Gene ID: (Human) 2526
