Invitrogen

SSEA1 Monoclonal Antibody (MC-480)

SSEA1 Antibody in Immunocytochemistry (ICC/IF)

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SSEA1 Antibody (41-1200) in ICC/IF

Indirect immunofluorescent analysis of SHEFF4 human ES cells using Zymed Ms anti-SSEA-1 (Product # 41-1200). Image courtesy of Peter Andrews, Ph.D., University of Sheffield, Sheffield, UK.

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SSEA1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of CD15 was observed in THP-1 in comparison to low to negative in Ramos using SSEA1 Monoclonal Antibody (MC-480) (Product # 41-1200). {RE}

产品信息

41-1200

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-3 µg/mL

免疫组化 (IHC)

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免疫细胞化学 (ICC/IF)

Assay-dependent

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流式细胞分析 (Flow)

Assay-dependent

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放射免疫测定 (RIA)

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产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgM

分类

Monoclonal

类型

Antibody

克隆号

MC-480

抗原

X-irradiated F9 teratocarcinoma stem cells

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533496

靶标信息

CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Differentiation and molecular characterization of endothelial progenitor and vascular smooth muscle cells from induced pluripotent stem cells.

BioImpacts : BI

Dastouri M,Ozdag H,Akar AR,Can A

41-1200 was used in Immunocytochemistry to differentiate induced pluripotent stem cells into endothelial progenitor cells and VSMCs using a step-wise technique.

Thu Aug 31 00:00:00 UTC 2023

A Stem Cell Surge During Thyroid Regeneration.

Frontiers in endocrinology

Ma R,Morshed SA,Latif R,Davies TF

41-1200 was used in Immunohistochemistry, Flow Cytometry to explore the mechanism behind thyroid stem cell and progenitor cells in the regeneration and repair process after injury.

Tue May 25 00:00:00 UTC 2021

A Stem Cell Surge During Thyroid Regeneration.

Frontiers in endocrinology

Ma R,Morshed SA,Latif R,Davies TF

41-1200 was used in Immunohistochemistry, Flow Cytometry to explore the mechanism behind thyroid stem cell and progenitor cells in the regeneration and repair process after injury.

Tue May 25 00:00:00 UTC 2021

Resveratrol enhances pluripotency of mouse embryonic stem cells by activating AMPK/Ulk1 pathway.

Cell death discovery

Suvorova II,Knyazeva AR,Petukhov AV,Aksenov ND,Pospelov VA

41-1200 was used in Flow cytometry/Cell sorting to study the effect of resveratrol on proliferation and pluripotency of mouse embryonic stem cells.

Mon Sep 28 00:00:00 UTC 2020

Mouse iPSC generated with porcine reprogramming factors as a model for studying the effects of non-silenced heterologous transgenes on pluripotency.

Journal of stem cells & regenerative medicine

Petkov SG,Glage S,Niemann H

41-1200 was used in Immunocytochemistry to evaluate the ability of transposon-based vectors with porcine pluripotency factors to reprogram mouse foetal fibroblasts to pluripotency.

Wed Sep 30 00:00:00 UTC 2020

G1 checkpoint is compromised in mouse ESCs due to functional uncoupling of p53-p21Waf1 signaling.

Cell cycle (Georgetown, Tex.)

Suvorova II,Grigorash BB,Chuykin IA,Pospelova TV,Pospelov VA

41-1200 was used in immunocytochemistry to determine the role of uncoupling of p53/p21-Waf1 signaling and G1 checkpoint disruption in mouse ESCs

Thu Oct 13 00:00:00 UTC 2016

Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens.

Stem cells (Dayton, Ohio)

Henderson JK,Draper JS,Baillie HS,Fishel S,Thomson JA,Moore H,Andrews PW

Wed Jan 08 00:00:00 UTC 2003

Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens.

Stem cells (Dayton, Ohio)

Henderson JK,Draper JS,Baillie HS,Fishel S,Thomson JA,Moore H,Andrews PW

Wed Jan 08 00:00:00 UTC 2003

Comparative analysis of cell surface antigens expressed by cell lines derived from human germ cell tumours.

International journal of cancer

Andrews PW,Casper J,Damjanov I,Duggan-Keen M,Giwercman A,Hata J,von Keitz A,Looijenga LH,Millán JL,Oosterhuis JW,Pera M,Sawada M,Schmoll HJ,Skakkebaek NE,van Putten W,Stern P

Tue Jun 11 00:00:00 UTC 1996

Comparative analysis of cell surface antigens expressed by cell lines derived from human germ cell tumours.

International journal of cancer

Andrews PW,Casper J,Damjanov I,Duggan-Keen M,Giwercman A,Hata J,von Keitz A,Looijenga LH,Millán JL,Oosterhuis JW,Pera M,Sawada M,Schmoll HJ,Skakkebaek NE,van Putten W,Stern P

Tue Jun 11 00:00:00 UTC 1996

Monoclonal antibody defining a stage-specific mouse embryonic antigen (SSEA-1).

Proceedings of the National Academy of Sciences of the United States of America

Solter D,Knowles BB

Wed Nov 01 00:00:00 UTC 1978

Monoclonal antibody defining a stage-specific mouse embryonic antigen (SSEA-1).

Proceedings of the National Academy of Sciences of the United States of America

Solter D,Knowles BB

Wed Nov 01 00:00:00 UTC 1978

生物信息学

蛋白别名: 3-fucosyl-N-acetyl-lactosamine epitope; 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase; alpha (1,3) fucosyltransferase, myeloid specific; alpha (1,3) fucosyltransferase, myeloid-specific; alpha 1,3-fucosyltransferase; Alpha-(1,3)-fucosyltransferase 4; ELAM ligand fucosyltransferase; ELAM-1 ligand fucosyltransferase; Fuc-TIV; Fucosyltransferase 4; fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific); Fucosyltransferase IV; fucT-IV; FUTIV; Galactoside 3-L-fucosyltransferase; Lewis X; SSEA-1; stage-specific embryonic antigen 1

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基因别名: AI451562; CD15; ELFT; FAL; FCT3A; FUC-TIV; FucT-IV; FUT4; FUTIV; LeX; SSEA-1; Ssea1

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UniProt ID: (Human) P22083, (Mouse) Q11127

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Entrez Gene ID: (Human) 2526, (Mouse) 14345

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功能