Invitrogen

SSTR3 Recombinant Rabbit Monoclonal Antibody (8H32L3)

SSTR3 Antibody in Immunocytochemistry (ICC/IF)

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SSTR3 Antibody (704006) in ICC/IF

HEK-293 cells stably transfected with SSTR3 were grown on poly-L-lysine-coated coverslips overnight. After 24 h, cells were fixed with 4% paraformaldehyde and 0.2% picric acid in phosphate buffer (pH 6.9) for 30 min at room temperature and washed several times with phosphate buffer. Specimens were permeabilized with ice-cold methanol and then incubated with SSTR3 Recombinant Rabbit Monoclonal Antibody (Product # 704006, 1:1,000 dilution). Cells were then incubated with Alexa488-conjugated secondary antibody for 2 h at room temperature, mou... View More

SSTR3 Antibody in Immunohistochemistry (IHC)

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产品信息

704006

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化 (IHC)

1:1,000

免疫细胞化学 (ICC/IF)

1:1,000

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

8H32L3

抗原

Peptide corresponding to the aa residues 398-418 of the human SSTR3

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_3073992

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

靶标信息

Expression of Somatostatin Receptor Type 3 (SSTR3) has been reported in brain, breast, eye, gastrointestinal tract, pancreas, and thyroid. G-protein Coupled Receptors (GPCRs) comprise one of the largest families of signaling molecules with more than a thousand members currently predicted to exist. All GPCRs share a structural motif consisting of seven membrane-spanning helices, and exist in both active and inactive forms. An array of activating ligands participate in the conformation of GPCRs which leads to signaling via G-proteins and downstream effectors. Ongoing studies have also shown the vast series of reactions which participate in the negative regulation of GPCRs. This "turn-off" activity has tremendous implications for the physiological action of the cell, and continues to drive pharmacological research for new drug candidates. Two blockbuster drugs which have been developed as GPCR-targeted pharmaceuticals are Zyprexa (Eli Lilly) and Claritin (Schering-Plough) which have multi-billion dollar shares of the mental health and allergy markets, respectively.

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