Invitrogen

ST2 Polyclonal Antibody

ST2 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 13

ST2 Antibody (PA5-20077) in ICC/IF

Immunofluorescence of ST2 in Mouse Kidney cells with ST2 Polyclonal Antibody (Product # PA5-20077) at 20 µg/mL.

ST2 Antibody in Immunohistochemistry (IHC)

ST2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

ST2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of Interleukin-1 receptor-like 1 was observed as increased expression in cardiomyocyte differentiated iPSCs and K-562 as compared to in U-937 using Anti-ST2 Polyclonal Antibody (Product # PA5-20077) in Western Blot. {RE}

产品信息

PA5-20077

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

查看1篇已发表文章 1篇已发表文章

免疫组化 (IHC)

5 µg/mL

查看5篇已发表文章 5篇已发表文章

免疫组化（石蜡） (IHC (P))

查看2篇已发表文章 2篇已发表文章

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

5 µg/mL

流式细胞分析 (Flow)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to 16 amino acids at the amino-terminus of mouse ST2

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

0.02% sodium azide

保存条件

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_11156630

产品详细信息

The immunogenic sequence is common to all three known ST2 isoforms.

A suggested positive control is 293 cell lysate.

PA5-20077 can be used with blocking peptide PEP-0195.

靶标信息

IL-33R, also known as ST2, is a receptor for IL-33. It is a member of the IL-1 receptor family of innate receptors. Its extracellular domain consists of 3 Ig-like domains that directly interact with its co-receptor IL-1RAcP, and the ligand alarmin, IL-33. IL-33 ligation results in the association of IL-33R complex with its adaptor proteins MyD88, IRAK1, IRAK4 and TRAF6, and activation of the NF-kB, and MAPK pathways. This, in turn, instigates the release of chemokines and cytokines such as IL-5, IL-6, IL-8, and IL-13. Two most common isoforms of IL-33R that result from alternative splicing are ST2L - the membrane bound form, and ST2S - the soluble form. The membrane bound form can be released from the cell surface by proteolytic cleavage. The soluble forms have been suggested to act as decoys dampening the IL-33 signaling. IL-33R is primarily expressed by mast cells, basophils, and eosinophils. In mouse, IL-33R expression has been shown on ILC2 and Th2 cells. IL-33R signaling has been implicated in the number of immune conditions, including allergies, arthritis, atherosclerosis and sepsis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Group 2 innate lymphoid cells protect mouse heart from myocardial infarction injury via interleukin 5, eosinophils, and dendritic cells.

Cardiovascular research

Liu T,Meng Z,Liu J,Li J,Zhang Y,Deng Z,Luo S,Wang M,Huang Q,Zhang S,Fendt P,Devouassoux J,Li D,McKenzie ANJ,Nahrendorf M,Libby P,Guo J,Shi GP

PA5-20077 was used in Immunohistochemistry to reveal an indirect cardiac reparative role of ILC2 in post-MI hearts via the IL5, EOS, and DC mechanism.

Tue May 02 00:00:00 UTC 2023

Group 2 Innate Lymphoid Cells Protect Mice from Abdominal Aortic Aneurysm Formation via IL5 and Eosinophils.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhang Y,Liu T,Deng Z,Fang W,Zhang X,Zhang S,Wang M,Luo S,Meng Z,Liu J,Sukhova GK,Li D,McKenzie ANJ,Libby P,Shi GP,Guo J

PA5-20077 was used in Immunohistochemistry to establish both direct and indirect roles for ILC2 in peri-aortic CaPO4 injury-induced mouse abdominal aortic aneurysms.

Wed Mar 01 00:00:00 UTC 2023

Type 2 Innate Lymphoid Cells Accumulate in the Brain After Hypoxia-Ischemia but Do Not Contribute to the Development of Preterm Brain Injury.

Frontiers in cellular neuroscience

Zelco A,Rocha-Ferreira E,Nazmi A,Ardalan M,Chumak T,Nilsson G,Hagberg H,Mallard C,Wang X

PA5-20077 was used in Immunohistochemistry-immunofluorescence to investigate if innate lymphoid cells, especially ILC2s, play a role in preterm brain injury.

Sat Apr 16 00:00:00 UTC 2022

Type 2 Innate Lymphoid Cells Accumulate in the Brain After Hypoxia-Ischemia but Do Not Contribute to the Development of Preterm Brain Injury.

Frontiers in cellular neuroscience

Zelco A,Rocha-Ferreira E,Nazmi A,Ardalan M,Chumak T,Nilsson G,Hagberg H,Mallard C,Wang X

PA5-20077 was used in Immunohistochemistry-immunofluorescence to investigate if innate lymphoid cells, especially ILC2s, play a role in preterm brain injury.

Sat Apr 16 00:00:00 UTC 2022

Spinal IL-33/ST2 signaling mediates chronic itch in mice through the astrocytic JAK2-STAT3 cascade.

Glia

Du L,Hu X,Yang W,Yasheng H,Liu S,Zhang W,Zhou Y,Cui W,Zhu J,Qiao Z,Maoying Q,Chu Y,Zhou H,Wang Y,Mi W

PA5-20077 was used in Western Blotting to reveal that spinal IL-33/ST2 signaling pathway contributes to chronic itch via astrocytic JAK2-STAT3 cascade activation, promoting TNF-α release to regulate the GRP/GRPR signaling-related itch response.

Sun Sep 01 00:00:00 UTC 2019

Spinal IL-33/ST2 signaling mediates chronic itch in mice through the astrocytic JAK2-STAT3 cascade.

Glia

Du L,Hu X,Yang W,Yasheng H,Liu S,Zhang W,Zhou Y,Cui W,Zhu J,Qiao Z,Maoying Q,Chu Y,Zhou H,Wang Y,Mi W

Sun Sep 01 00:00:00 UTC 2019

Epidermal Growth Factor Receptor Expression Licenses Type-2 Helper T Cells to Function in a T Cell Receptor-Independent Fashion.

Immunity

Minutti CM,Drube S,Blair N,Schwartz C,McCrae JC,McKenzie AN,Kamradt T,Mokry M,Coffer PJ,Sibilia M,Sijts AJ,Fallon PG,Maizels RM,Zaiss DM

Published figure using ST2 polyclonal antibody (Product # PA5-20077) in Flow Cytometry

Tue Oct 17 00:00:00 UTC 2017

Expression of interleukin-33 and its receptor ST2 in periapical granulomas and radicular cysts.

Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology

Velickovic M,Pejnovic N,Petrovic R,Mitrovic S,Jeftic I,Kanjevac T,Lukic A

PA5-20077 was used in immunohistochemistry - frozen section to determine the expression patterns of IL-33 and ST2 in human periapical lesions

Fri Jan 01 00:00:00 UTC 2016

Deletion of IL-33R attenuates VEGF expression and enhances necrosis in mammary carcinoma.

Oncotarget

Milosavljevic MZ,Jovanovic IP,Pejnovic NN,Mitrovic SL,Arsenijevic NN,Simovic Markovic BJ,Lukic ML

PA5-20077 was used in immunohistochemistry - paraffin section to study the attenuation of VEGF expression and enhancement of necrosis in mammary carcinoma by deletion of IL-33R

Tue Apr 05 00:00:00 UTC 2016

Deletion of IL-33R attenuates VEGF expression and enhances necrosis in mammary carcinoma.

Oncotarget

Milosavljevic MZ,Jovanovic IP,Pejnovic NN,Mitrovic SL,Arsenijevic NN,Simovic Markovic BJ,Lukic ML

PA5-20077 was used in immunohistochemistry - paraffin section to study the attenuation of VEGF expression and enhancement of necrosis in mammary carcinoma by deletion of IL-33R

Tue Apr 05 00:00:00 UTC 2016

ST2 deletion increases inflammatory bone destruction in experimentally induced periapical lesions in mice.

Journal of endodontics

Velickovic M,Pejnovic N,Mitrovic S,Radosavljevic G,Jovanovic I,Kanjevac T,Jovicic N,Lukic A

PA5-20077 was used in immunohistochemistry - paraffin section to investigate the role of ST2 signaling in the pathogenesis of periapical lesions

Sun Mar 01 00:00:00 UTC 2015

生物信息学

蛋白别名: growth stimulation-expressed; homolog of mouse growth stimulation-expressed; IL-1 R4; IL-1RL1; IL-33R; interleukin 1 receptor-like 1; interleukin 1 receptor-related protein; Interleukin-1 receptor-like 1; Interleukin-33 receptor alpha chain; Interleukin1 receptor like 1; Lymphocyte antigen 84; Protein ST2; Protein T1; sIL 33R; soluble IL 33 Receptor; soluble IL 33R

查看更多收起

基因别名: DER4; FIT-1; IL1RL1; IL33R; Ly84; ST2; St2-rs1; ST2L; ST2V; Ste2; T1; T1/ST2

查看更多收起

UniProt ID: (Human) Q01638, (Mouse) P14719

查看更多收起

Entrez Gene ID: (Human) 9173, (Mouse) 17082

查看更多收起

功能

interleukin-33 binding interleukin-33 receptor activity cytokine receptor activity interleukin-1 receptor activity receptor signaling protein activity protein binding

参与通路