Rockland

STIM1 Polyclonal Antibody

STIM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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STIM1 Antibody (600-401-J48) in IHC (P)

Immunohistochemistry of Rabbit anti-GOK / STIM1 antibody. Tissue: Testis. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: GOK / STIM1 antibody at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: GOK / STIM1 as precipitated red signal with hematoxylin purple nuclear counterstain.

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产品信息

600-401-J48

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

免疫组化（石蜡） (IHC (P))

5 µg/mL

酶联免疫吸附实验 (ELISA)

1: 10,000

免疫沉淀 (IP)

1 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Stim 1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the C-terminus of human Stim 1.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.03 mg/mL

纯化类型

Affinity chromatography

保存液

0.02M potassium phosphate, pH 7.2, with 50% glycerol, 0.15M NaCl, 1mg/mL BSA

内含物

no preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

A BLAST analysis was used to suggest cross-reactivity with rat, human, bovine, and mouse based on 100% sequence homology. Cross-reactivity with Stim I from other sources has not been determined.

靶标信息

STIM1 (stromal interacting molecule; also known as GOK) acts as a sensor of calcium depletion within the endoplasmic reticulum and transduces the signal to Orai1, the presumptive CRAC channel at the plasma membrane. Following decrease of luminal calcium concentration, STIM1 oligomerizes and induces Orai1 to enable entry of extracellular calcium into the cytoplasm. However, the precise mechanism of STIM1-Orai1 interaction has not been elucidated yet. Many questions also remain to be solved around STIM1 functional distribution. It turns out that STIM1 associates with growing ends of microtubules and is involved in endoplasmic reticulum tubule extension.

仅用于科研。不用于诊断过程。未经明确授权不得转售。