Invitrogen

STIM1 Polyclonal Antibody

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STIM1 Antibody (PA1-46217) in WB

Western blot was performed using Anti-STIM1 Polyclonal Antibody (Product # PA1-46217) and bands corresponding to 83 kDa and 77 kDa of STIM1 were observed across all the cell lines and tissues tested along with an uncharacterized band (*) at ~50 kDa across the panel. Whole cell extracts (30 µg lysate) of PC-12 (Lane 1), MCF 10A (Lane 2), MCF7 (Lane 3), MIA PaCa-2 (Lane 4), PANC-1 (Lane 5), tissue extracts (30 µg lysate) of Mouse Brain (Lane 6) ad Mouse Skeletal Muscle (Lane 7) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Pro... View More

STIM1 Antibody in Immunocytochemistry (ICC/IF)

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产品信息

PA1-46217

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫细胞化学 (ICC/IF)

1:50-1:200

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产品规格

种属反应

Bovine, Dog, Human, Mouse, Rat

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide made to a C-terminal region with residues 600-685 of the human protein.[Uniprot#Q13586]

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 30% glycerol

内含物

0.1% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2197881

产品详细信息

The target sequence has 93% sequence homology with porcine and 79% sequence homology with xenopus.

Suggested positive control: antigen standard for STIM1 (transient overexpression lysate), HeLa whole cell lysate.

靶标信息

STIM1 (stromal interacting molecule; also known as GOK) acts as a sensor of calcium depletion within the endoplasmic reticulum and transduces the signal to Orai1, the presumptive CRAC channel at the plasma membrane. Following decrease of luminal calcium concentration, STIM1 oligomerizes and induces Orai1 to enable entry of extracellular calcium into the cytoplasm. However, the precise mechanism of STIM1-Orai1 interaction has not been elucidated yet. Many questions also remain to be solved around STIM1 functional distribution. It turns out that STIM1 associates with growing ends of microtubules and is involved in endoplasmic reticulum tubule extension.

仅用于科研。不用于诊断过程。未经明确授权不得转售。